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On the Role of Escherichia coli DNA Polymerase I and of T4 Gene 32 Protein in Recombination of Phage T4

  • Gisela Mosig

Abstract

As discussed repeatedly during this symposium, recombination between DNA molecules proceeds in several steps (see Fig. 1): (A) Breakage or nicking of parental molecules (Meselson and Weigle, 1961), (B) formation of “joint” intermediates (Anraku and Tomizawa, 1965), some of them branched (Broker and Lehman, 1971; Benbow et al., this symposium), (C) conversion of “joint” into covalently linked “recombinant” molecules, which may still contain mismatched base pairs in the heteroduplex regions (Tomizawa, 1967; Kozinski et al., 1967), (D) repair of mismatches in heteroduplex regions (Spatz and Trautner, 1970; Fox, Meselson, this symposium), and (E) replication of “recombinant” DNA molecules, which resolves unrepaired mismatches in heteroduplex regions. The temporal sequence of some of these steps is not well defined; i.e., breakage may precede or follow the formation of “joint” molecules and repair may precede or follow the conversion of “joint” into “recombinant” molecules.

Keywords

Genetic Recombination Covalent Linkage Negative Interference Positive Interference Nucleolytic Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Alberts, B. M. and L. Frey. 1970. T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA. Nature 227: 1313.PubMedCrossRefGoogle Scholar
  2. Anraku, N., Y. Anraku and I. R. Lehman. 1969. Enzymic joining of polynucleotides VIII. Structure of hybrids of parental T4 DNA molecules. J. Mol. Biol. 46: 481.PubMedCrossRefGoogle Scholar
  3. Anraku, N. and J. Tomizawa. 1965. Molecular mechanisms of genetic recombination of bacteriophage V. Two kinds of joining of parental DNA molecules. J. Mol. Biol. 12: 805.PubMedCrossRefGoogle Scholar
  4. Broker, T. R. 1973. An electron microscopic analysis of pathways for bacteriophage T4 DNA recombination. J. Mol. Biol. 81: 1.PubMedCrossRefGoogle Scholar
  5. Broker, T. R. and I. R. Lehman. 1971. Branched DNA molecules: Intermediates in T4 recombination. J. Mol. Biol. 60: 131.PubMedCrossRefGoogle Scholar
  6. Childs, J. D. 1971. A map of molecular distances between mutations of bacteriophge T4D. Genetics 67: 455.PubMedGoogle Scholar
  7. Doermann, A. H. and D. H. Parma. 1967. Recombination in bacteriophage T4. J. Cell. Physiol. 70 (Suppl. 1): 147.PubMedCrossRefGoogle Scholar
  8. Ennis, H. L. and K. D. Kievitt. 1973. Association of the rIIA protein with the bacterial membrane. Proc. Nat. Acad. Sci. U.S.A. 70: 1468.CrossRefGoogle Scholar
  9. Fox, M. S. 1966. On the mechanism of integration of transforming deoxyribonucleate. J. Gen. Physiology 49: 183.CrossRefGoogle Scholar
  10. Heijneker, H. L., D. J. Ellens, R. H. Tjeerde, B. W. Glickman, B. van Dorp and P. H. Pouwels. 1973. A mutant of Escherichia coli K12 deficient in the 5′-3′ exonucleolytic activity of DNA polymerase I. II. Purification and properties of the mutant enzyme. Molec. Gen. Genet. 124: 83.PubMedCrossRefGoogle Scholar
  11. Holliday, R. 1964. A mechanism for gene conversion in fungi. Genet. Res. 5: 282.CrossRefGoogle Scholar
  12. Hotchkiss, R. D. 1971. Toward a general theory of genetic recombination in DNA. Advan. Genet. 16: 325.CrossRefGoogle Scholar
  13. Huberman, J. A., A. Kornberg and B. M. Alberts. 1971. Stimulation of T4 bacteriophage DNA polymerase by the protein product of T4 gene 32. J. Mol. Biol. 62: 39.PubMedCrossRefGoogle Scholar
  14. Kozinski, A. and Z. Felgenhauer. 1967. Molecular recombination in T4 bacteriophage deoxyribonucleic acid II. Single-strand breaks and exposure of uncomplemented areas as a prerequisite for recombination. J. Virol. 1: 1193.PubMedGoogle Scholar
  15. Kozinski, A. W., P. B. Kozinski and R. James. 1967. Molecular recombination in T4 bacteriophage deoxyribonucleic acid I. Tertiary structure of early replicative and recombining deoxyribonucleic acid. J. Virol. 1: 758.PubMedGoogle Scholar
  16. Lee, C. S., R. W. Davis and N. Davidson. 1970. A physical study by electron microscopy of the terminally repetitious, circularly permuted DNA from the coliphage particles of Esohevichia coli 15. J. Mol. Biol. 48: 1.PubMedCrossRefGoogle Scholar
  17. Lehman, I. R. and J. R. Chien. 1973. DNA polymerase I activity in polymerase I mutants of Escherichia coli. In (R. D. Wells and R. B. Inman, eds.) DNA Synthesis In Vitro, p. 3–12. University Park Press, Baltimore.CrossRefGoogle Scholar
  18. Majumdar, C., M. Dewey and F. R. Frankel. 1972. Bacteriophage-directed association of DNA polymerase I with host membrane: A dispensable function. Virology 49: 134.PubMedCrossRefGoogle Scholar
  19. Meselson, M. 1972. Formation of hybrid DNA by rotary diffusion during genetic recombination. J. Mol. Biol. 71: 795.PubMedCrossRefGoogle Scholar
  20. Meselson, M. and J. J. Weigle. 1961. Chromosome breakage accompanying genetic recombination in bacteriophage. Proc. Nat. Acad. Sci. U.S.A. 47: 857.CrossRefGoogle Scholar
  21. Monk, M. and J. Kinross. 1972. Conditional lethality of rec A and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic acid polymerase I. J. Bacteriology 109: 971.Google Scholar
  22. Mosig, G. 1963. Genetic recombination in bacteriophage T4 during replication of DNA fragments. Cold Spring Harbor Symp. Quant. Biol. 28: 35.CrossRefGoogle Scholar
  23. Mosig, G. 1968. A map of distances along the DNA molecule of phage T4. Genetics 59: 137.PubMedGoogle Scholar
  24. Mosig, G., D. W. Bowden and S. Bock. 1972a. E. coli DNA polymerase I and other host functions participate in T4 DNA replication and recombination. Nature New Biol. 240: 12.PubMedGoogle Scholar
  25. Mosig, G., J. R. Carnighan, J. B. Bibring, R. Cole, H-G. O. Bock and S. Bock. 1972b. Coordinate variation in lengths of deoxyribonucleic acid molecules and head lengths in morphological variants of bacteriophage T4. J. Virol. 9: 857.PubMedGoogle Scholar
  26. Mosig, G., R. Ehring, W. Schliewen and S. Bock. 1971. The patterns of recombination and segregation in terminal regions of T4 DNA molecules. Molec. Gen. Genet. 113: 51.PubMedGoogle Scholar
  27. Parma, D. H. 1969. The structure of genomes of individual petit particles of the bacteriophage T4D mutant E920/96/41. Genetics 63: 247.PubMedGoogle Scholar
  28. Sigal, N. and B. Alberts. 1972. Genetic recombination: the nature of a crossed strand-exchange between two homologous DNA molecules. J. Mol. Biol. 71: 789.PubMedCrossRefGoogle Scholar
  29. Spatz, H. C. and T. A. Trautner. 1970. One way to do experiments on gene conversion? Transfection with heteroduplex SPP1 DNA. Molec. Gen. Genet. 109: 84.Google Scholar
  30. Tomizawa, J-I. 1967. Molecular mechanisms of genetic recombination in bacteriophage: Joint molecules and their conversion to recombinant molecules. J. Cell Physiol. 70: 201.PubMedCrossRefGoogle Scholar
  31. Weintraub, S. B. and F. R. Frankel. 1972. Identification of the T4 rIIB gene product as a membrane protein. J. Mol Biol. 10: 589.CrossRefGoogle Scholar
  32. Whitehouse, H. L. K. 1963. A theory of crossing-over by means of hybrid deoxyribonucleic acid. Nature 199: 1034.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Gisela Mosig
    • 1
  1. 1.Vanderbilt UniversityNashvilleUSA

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