The Isolation of Mitotic rec- Mutants in Schizosaccharomyces pombe
The mechanisms governing the interaction of homologous DNA molecules that lead to the formation of genetic recombinants remain largely unknown in eukaryotes. Recent evidence (for review see Stern and Hotta, 1973) obtained from work with several species of Lilium indicates that the enzymatic control of recombination may be similar to that observed in bacteria and viruses. Given the fact that a portion of the genome directs the synthesis of enzymes that mediate recombination, additional information relating to its biochemistry could be obtained by selecting for mutants that alter genetic map distances. Presumably such an altered phenotype, e.g. absence of genetic recombination, would arise as a consequence of a modified or missing gene product that mediates exchange.
KeywordsMating Type Schizosaccharomyces Pombe Intragenic Recombination Minimal Agar Ade6 Locus
Unable to display preview. Download preview PDF.
- Catcheside, D. G. 1966. A second gene controlling allelic recombination in Neurospora crassa. Aust. J. Biol. Sci. 19: 1039.Google Scholar
- Catcheside, D. G. 1968. The control of genetic recombination in Neurospora crassa. In (W. J. Peacock and R. D. Brock, eds.) Replication and Recombination of Genetic Material pp. 216–226. Australian Acad. Sci., Canberra.Google Scholar
- Flores da Cunha, M. 1970. Mitotic mapping of Schizosaccharomyces pombe. Genet. Res. 16: 121.Google Scholar
- Gutz, H. 1963. Untersuchungen zur Feinstruktur der Gene ade7 and ade6 von Schizosaccharomyces pombe Lind. Habilitationsschrift, Technische Universität, Berlin.Google Scholar
- Strauss, B. 1969. DNA repair mechanisms and their relation to mutation and recombination. In Curr. Top. Microbiol. Immunol. 44: 1.Google Scholar