Lack of Correspondence between Genetic and Physical Distances in the Iso-1-Cytochrome c Gene of Yeast
It is necessary to study intragenic recombination at a locus for which the primary sequence analysis of the gene product is available if one wishes an unequivocal check on the positions of the mutant sites in the structural gene, as well as to uncover factors other than distance that might affect frequencies of genetic exchanges. The iso-1-cytochrome c gene of the bakers’ yeast Saccharomyces cerevisiae, therefore, is particularly suitable. Nucleotide sequences of numerous mutant codons have been identified from alterations in functional iso-1-cytochromes c produced by intragenic revertants, but all too often an order of cycl mutational sites establishable from mapping frequencies is not compatible with corresponding alterations in the sequence of amino-acid residues.
KeywordsPhysical Distance Mutant Codon Haploid Strain Intragenic Recombination Deletion Mapping
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