Role of a Deoxyribonuclease in Bacterial Transformation

  • Sanford Lacks


In the genetic transformation of Diplococcus pneumoniae, donor DNA is converted to single strands either during or just after its uptake by the cell (Lacks, 1962). This conversion may be regarded as the first step in preparation of the DNA for recombination, since a single-strand segment of donor DNA is ultimately inserted into recipient cell DNA (Fox and Allen, 1964). In addition to its role in the recombination process, this step may be an essential part of the mechanism for DNA entry.


Genetic Transformation Brookhaven National Laboratory DNase Activity Defective Mutant Bacterial Transformation 
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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Sanford Lacks
    • 1
  1. 1.Biology DepartmentBrookhaven National LaboratoryUptonUSA

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