In Vitro Transformation in Toluenized Bacillus subtilis
Studies on bacterial transformation have contributed to the understanding of the physical state of transforming DNA in recipient cells. In the early stage of transformation a double-stranded DNA with low transforming activity was found in periplasm of Bacillus subtilis (Dubnau and Davidoff-Abelson, 1971). Single-stranded intermediates were found in Pneumococcus (Lacks, 1962) and in B. subtilis (Piechowska and Fox, 1971; Davidoff-Abelson and Dubnau, 1973a) which were later found as heteroduplex donor-recipient complexes in Pneumococcus, Haemophilus, and B. subtilis (Lacks, 1962; Fox and Allen, 1964; Notani and Goodgal, 1966; Davidoff-Abelson and Dubnau, 1973b). However, the molecular mechanisms of transformation, which may involve enzyme(s), cellular structure(s), and other factors, still remain obscure. In this paper we report on transformation studies in toluene-treated B. subtilis, in which DNA, protein, and RNA synthesis in the presence of proper substrates and ATP has been shown to occur.
KeywordsBacillus Subtilis Recipient Cell Transforming Activity Original Culture Bacterial Transformation
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