Uptake and Integration of Transforming DNA in Bacillus Subtilis
In the past decade, considerable information has accumulated concerning the molecular events that accompany the uptake and integration of transforming DNA by competent bacterial cultures. This article presents our understanding of the molecular events accompanying genetic transformation in Bacillus subtilis. The approach employed in our laboratory has been a straightforward one, involving the addition of isotopically and genetically labeled DNA to competent cells and the analysis of extracts of those cells.
KeywordsBacillus Subtilis Deoxyribonucleic Acid Donor Segment Donor Moiety polA59 Strain
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