Transformation-Mediated Recombination in Escherichia coli
Recently generalized and specialized transformation systems in an Escherichia coli K12 strain have been established (Oishi and Cosloy, 1972, 1974; Cosloy and Oishi, 1973a,b; Wackernagel, 1973). Among various factors that affect the frequency of transformation, the status of the recombination pathways in the recipient cells, especially that of recBrecC DNase (ATP-dependent DNase), seemed to have the most striking effect on the transformation. We report here some of the basic characteristics of the transformation system in E. coli, with special emphasis on the recombination process involved, and discuss the effect of recBrecC DNase on the mode of genetic recombination. (The effect of recBrecC Dnase on transformation and transduction is also described by Wackernagel and Radding in this volume.)
KeywordsTransformation Frequency Recipient Cell Genetic Recombination Specialized Transformation Recombination Pathway
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