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Isolation of the Enzyme Associated with the sbcA Indirect Suppressor

  • Sidney R. Kushner
  • Haruko Nagaishi
  • A. J. Clark

Abstract

recB and/or recC deficiency in Escherichia coli K-12 is indirectly suppressed in the presence of sbcA - mutations. Such strains remain genotypically recB and/or recC deficient but become recombination proficient and resistant to ultraviolet light. Barbour et al. (1970) showed that strains carrying sbcA - mutations contained increased levels of an ATP-independent DNase. Since the product of the recB-recC genes (exonuclease V) is a multifunctional DNase that requires ATP for the hydrolysis of double-stranded DNA (Goldmark and Linn, 1972), characterization of the ATP-independent nuclease found in sbcA - strains might provide an important clue to the in vivo function of the recB-recC gene products.

Keywords

Ammonium Sulfate Crude Lysate Public Health Service Research Strain JC7693 Lambda Exonuclease 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Barbour, S. D., H. Nagaishi, A. Templin and A. J. Clark. 1970. Biochemical and genetic studies of recombination proficiency in Escherichia coli II. Rec + revertants caused by indirect suppression of rec - mutations. Proc. Nat. Acad. Sci. U.S.A. 67: 128.CrossRefGoogle Scholar
  2. Goldmark, P. J. and S. Linn. 1972. Purification and properties of the recBC DNase of Escherichia coli. J. Biol. Chem. 247: 1849.PubMedGoogle Scholar
  3. Kushner, S. R., H. Nagaishi and A. J. Clark. 1974. Isolation of exonuclease VIII: The enzyme associated with the sbcA indirect suppressor. Proc. Nat. Acad. Sci. U.S.A. in press.Google Scholar
  4. Richardson, C. C. and A. Kornberg. 1964. A deoxyribonucleic acid phosphatase-exonuclease from Escherichia coli. I. Purification of the enzyme and characterization of the phosphatase activity. J. Biol. Chem. 239: 242.PubMedGoogle Scholar
  5. Richardson, C. C., C. L. Schildkraut, H. V. Aposhian and A. Kornberg. 1964. Enzymatic synthesis of deoxyribonucleic acid. XIV. Further purification and properties of deoxyribonucleic acid polymerase of Escherichia coli. J. Biol. Chem. 239: 222.PubMedGoogle Scholar
  6. Templin, A., S. R. Kushner and A. J. Clark. 1972. Genetic analysis of mutations indirectly suppressing recB and recC mutations. Genetics 72: 205.Google Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Sidney R. Kushner
    • 1
  • Haruko Nagaishi
    • 2
  • A. J. Clark
    • 2
  1. 1.Department of BiochemistryStanford University School of MedicineStanfordUSA
  2. 2.Department of Molecular BiologyUniversity of CaliforniaBerkeleyUSA

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