Isolation of the Enzyme Associated with the sbcA Indirect Suppressor
recB and/or recC deficiency in Escherichia coli K-12 is indirectly suppressed in the presence of sbcA - mutations. Such strains remain genotypically recB and/or recC deficient but become recombination proficient and resistant to ultraviolet light. Barbour et al. (1970) showed that strains carrying sbcA - mutations contained increased levels of an ATP-independent DNase. Since the product of the recB-recC genes (exonuclease V) is a multifunctional DNase that requires ATP for the hydrolysis of double-stranded DNA (Goldmark and Linn, 1972), characterization of the ATP-independent nuclease found in sbcA - strains might provide an important clue to the in vivo function of the recB-recC gene products.
KeywordsAmmonium Sulfate Crude Lysate Public Health Service Research Strain JC7693 Lambda Exonuclease
Unable to display preview. Download preview PDF.
- Kushner, S. R., H. Nagaishi and A. J. Clark. 1974. Isolation of exonuclease VIII: The enzyme associated with the sbcA indirect suppressor. Proc. Nat. Acad. Sci. U.S.A. in press.Google Scholar
- Templin, A., S. R. Kushner and A. J. Clark. 1972. Genetic analysis of mutations indirectly suppressing recB and recC mutations. Genetics 72: 205.Google Scholar