Abstract
recB and/or recC deficiency in Escherichia coli K-12 is indirectly suppressed in the presence of sbcA - mutations. Such strains remain genotypically recB and/or recC deficient but become recombination proficient and resistant to ultraviolet light. Barbour et al. (1970) showed that strains carrying sbcA - mutations contained increased levels of an ATP-independent DNase. Since the product of the recB-recC genes (exonuclease V) is a multifunctional DNase that requires ATP for the hydrolysis of double-stranded DNA (Goldmark and Linn, 1972), characterization of the ATP-independent nuclease found in sbcA - strains might provide an important clue to the in vivo function of the recB-recC gene products.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Barbour, S. D., H. Nagaishi, A. Templin and A. J. Clark. 1970. Biochemical and genetic studies of recombination proficiency in Escherichia coli II. Rec + revertants caused by indirect suppression of rec - mutations. Proc. Nat. Acad. Sci. U.S.A. 67: 128.
Goldmark, P. J. and S. Linn. 1972. Purification and properties of the recBC DNase of Escherichia coli. J. Biol. Chem. 247: 1849.
Kushner, S. R., H. Nagaishi and A. J. Clark. 1974. Isolation of exonuclease VIII: The enzyme associated with the sbcA indirect suppressor. Proc. Nat. Acad. Sci. U.S.A. in press.
Richardson, C. C. and A. Kornberg. 1964. A deoxyribonucleic acid phosphatase-exonuclease from Escherichia coli. I. Purification of the enzyme and characterization of the phosphatase activity. J. Biol. Chem. 239: 242.
Richardson, C. C., C. L. Schildkraut, H. V. Aposhian and A. Kornberg. 1964. Enzymatic synthesis of deoxyribonucleic acid. XIV. Further purification and properties of deoxyribonucleic acid polymerase of Escherichia coli. J. Biol. Chem. 239: 222.
Templin, A., S. R. Kushner and A. J. Clark. 1972. Genetic analysis of mutations indirectly suppressing recB and recC mutations. Genetics 72: 205.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1974 Plenum Press, New York
About this chapter
Cite this chapter
Kushner, S.R., Nagaishi, H., Clark, A.J. (1974). Isolation of the Enzyme Associated with the sbcA Indirect Suppressor. In: Grell, R.F. (eds) Mechanisms in Recombination. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2133-0_13
Download citation
DOI: https://doi.org/10.1007/978-1-4684-2133-0_13
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-2135-4
Online ISBN: 978-1-4684-2133-0
eBook Packages: Springer Book Archive