Abstract
The redB and redX genes of bacteriophage λ specify a recombination pathway that efficiently recombines phage genomes in lytically infected recA- and recB- cells (see Signer, 1971, for a review). However, the red genes do not promote efficient specialized transduction of a recA- host (Manly et al., 1969; Mizuuchi and Fukasawa, 1969; Gottesman et al., 1974; H. Echols, personal communication). We report here that the red pathway does promote efficient transduction of redB- hosts.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Campbell, A. 1964. Transduction. In (I. C. Gunsalus and R. Y. Stanier, eds.) The Bacteria, vol. 5, p. 49. Academic Press, New York.
Gottesman, M. M., M. E. Gottesman, S. Gottesman and M. Geliert. 1974. Characterization of A reverse as an E. coli phage carrying a unique set of host derived recombination functions. J. Mol. Biol., submitted.
Little, J. and M. E. Gottesman. 1971. Defective lambda particles whose DNA carries only a single cohesive end. In (A. D. Hershey, ed.) The Bacteriophage A. p. 371. Cold Spring Harbor Laboratory, New York.
Manly, K., E. Signer and C. Radding. 1969. Nonessential functions of bacteriophage A. Virology 37: 177.
Mizuuchi, K. and T. Fukasawa. 1969. Chromosome mobilization in rec-merodiploids of Escherichia coli K12 following infection with bacteriophage A. Virology 39: 467.
Shimada, K., R. Weisberg and M. E. Gottesman. 1973. Prophage lambda at unusual chromosomal locations II. Mutations induced by bacteriophage lambda in Escherichia coli K12. J. Mol. Biol. 80: 297.
Signer, E. 1971. General recombination. In (A. D. Hershey, ed.) The Bacteriophage A. p. 139. Cold Spring Harbor Laboratory, New York.
Willets, N. and D. Mount. 1969. Genetic analysis of recombination-deficient mutants of Escherichia coli carrying rec mutations co-transducible with thyA. J. Bacteriol. 100: 405.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1974 Plenum Press, New York
About this chapter
Cite this chapter
Weisberg, R.A., Sternberg, N. (1974). Transduction of recB- Hosts is Promoted by λ red + Function. In: Grell, R.F. (eds) Mechanisms in Recombination. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2133-0_10
Download citation
DOI: https://doi.org/10.1007/978-1-4684-2133-0_10
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-2135-4
Online ISBN: 978-1-4684-2133-0
eBook Packages: Springer Book Archive