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Replication of the Single-Stranded DNA of Bacteriophage ϕX174 in Nucleotide-Permeable Cells

  • Ulrich Hess
  • Hans-Peter Vosberg
  • Hildegard Dürwald
  • Otto Schrecker
  • Hartmut Hoffmann-Berling

Abstract

The replication of φX174 is initiated by conversion of single-stranded ring DNA (SS) into a double-stranded replicative form (RF) DNA molecule. The fact that this conversion of SS to RF is insensitive to high concentration of chloramphenicol suggests involvement of pre-existing host enzyme(s) in this step. The product of a viral gene is required only for the next stage in which the RF replicates to yield further RF (Sinsheimer, 1968).

Keywords

Replicative Form Short Piece Defective Phage Alkaline Sucrose Gradient Velocity Sedimentation Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Ulrich Hess
    • 1
  • Hans-Peter Vosberg
    • 1
  • Hildegard Dürwald
    • 1
  • Otto Schrecker
    • 1
  • Hartmut Hoffmann-Berling
    • 1
  1. 1.Max-Planck-Institute für medizinische ForschungHeidelbergWest Germany

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