Replication of the Single-Stranded DNA of Bacteriophage ϕX174 in Nucleotide-Permeable Cells
The replication of φX174 is initiated by conversion of single-stranded ring DNA (SS) into a double-stranded replicative form (RF) DNA molecule. The fact that this conversion of SS to RF is insensitive to high concentration of chloramphenicol suggests involvement of pre-existing host enzyme(s) in this step. The product of a viral gene is required only for the next stage in which the RF replicates to yield further RF (Sinsheimer, 1968).
KeywordsReplicative Form Short Piece Defective Phage Alkaline Sucrose Gradient Velocity Sedimentation Analysis
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