The Joining of DNA Duplexes at their Base-Paired Ends

  • Vittorio Sgaramella
  • J. P. KennedyJr.


The enzymatic joining of two DNA duplexes can take place after their terminal single-stranded complementary sequences have formed hydrogen-bonded structures (cohesive joining). It is also possible to join DNA duplexes which have completely base-paired termini (terminal joining) in a reaction catalyzed by the T4 ligase. Fig. 1 summarizes the two modes of joining and this report describes some results obtained studying the terminal mechanism.


Chromosome Replication Terminal Nucleotide Hemophilus Influenzae Spleen Phosphodiesterase Sity Medical School 


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  3. Sgaramella, V. and H. G. Khorana. 1972. J. Mol. Biol. 72: 493.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Vittorio Sgaramella
    • 1
  • J. P. KennedyJr.
    • 2
  1. 1.Lab. CNR Genetica Biochimica ed EvoluzionisticaPaviaItaly
  2. 2.Lab. for Molecular Medicine, Genetics Dept.Stanford University Medical SchoolStanfordUSA

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