The Joining of DNA Duplexes at their Base-Paired Ends

  • Vittorio Sgaramella
  • J. P. KennedyJr.


The enzymatic joining of two DNA duplexes can take place after their terminal single-stranded complementary sequences have formed hydrogen-bonded structures (cohesive joining). It is also possible to join DNA duplexes which have completely base-paired termini (terminal joining) in a reaction catalyzed by the T4 ligase. Fig. 1 summarizes the two modes of joining and this report describes some results obtained studying the terminal mechanism.


Chromosome Replication Terminal Nucleotide Hemophilus Influenzae Spleen Phosphodiesterase Sity Medical School 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Sgaramella, V. 1972. Il Farmaco Internl. Ed. 27:809.Google Scholar
  2. Sgaramella, V. 1972. Proc. Nat. Acad. Sci., U.S.A. 69: 3389.CrossRefGoogle Scholar
  3. Sgaramella, V. and H. G. Khorana. 1972. J. Mol. Biol. 72: 493.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Vittorio Sgaramella
    • 1
  • J. P. KennedyJr.
    • 2
  1. 1.Lab. CNR Genetica Biochimica ed EvoluzionisticaPaviaItaly
  2. 2.Lab. for Molecular Medicine, Genetics Dept.Stanford University Medical SchoolStanfordUSA

Personalised recommendations