The Joining of DNA Duplexes at their Base-Paired Ends
The enzymatic joining of two DNA duplexes can take place after their terminal single-stranded complementary sequences have formed hydrogen-bonded structures (cohesive joining). It is also possible to join DNA duplexes which have completely base-paired termini (terminal joining) in a reaction catalyzed by the T4 ligase. Fig. 1 summarizes the two modes of joining and this report describes some results obtained studying the terminal mechanism.
KeywordsChromosome Replication Terminal Nucleotide Hemophilus Influenzae Spleen Phosphodiesterase Sity Medical School
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