The Role of ATP in Chromosome Replication Studied in Toluenized Escherichia coli

  • Patrick Forterre
  • Masamichi Kohiyama


Moses et al. (1970) have found an absolute ATP requirement, besides the four dNTP, for DNA replication in a toluene treated po1A 1 mutant. None of the known E. coli DNA polymerases are affected by ATP (Kornberg et al. 1970). Two major hypotheses can be considered to explain the role of ATP: ATP is a cofactor for some enzyme necessary to replication and/or it is a precursor for RNA synthesis involved in the priming of Okazaki pieces (Sugino et al. 1972).


Chromosome Replication Repair Synthesis Mutant CR341 
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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Patrick Forterre
    • 1
  • Masamichi Kohiyama
    • 1
  1. 1.Laboratoire des BiomembranesInstitut de Biologie MoleculaireParisFrance

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