The Role of ATP in Chromosome Replication Studied in Toluenized Escherichia coli
Moses et al. (1970) have found an absolute ATP requirement, besides the four dNTP, for DNA replication in a toluene treated po1A 1 − mutant. None of the known E. coli DNA polymerases are affected by ATP (Kornberg et al. 1970). Two major hypotheses can be considered to explain the role of ATP: ATP is a cofactor for some enzyme necessary to replication and/or it is a precursor for RNA synthesis involved in the priming of Okazaki pieces (Sugino et al. 1972).
KeywordsChromosome Replication Repair Synthesis Mutant CR341
Unable to display preview. Download preview PDF.