A Novel Form of DNA Appearing After Infection of Permissive Cells by SV40 Containing both Viral and Reiterated Host Sequences
Covalently linked SV40 and cellular RNA sequences have been described in productively infected cells (Jaenisch, 1972). In agreement with this observation integration of SV40 DNA into the host DNA of productively infected cells has been reported (Harai and Defendi, 1972). It was furthermore shown that integration of host DNA sequences into the mature SV40 superhelix occurs under conditions of high multiplicities of infection when virus pools are used which were serially passaged undiluted (Lavi and Winocour, 1972). This phenomenon could be explained by assuming integration of viral DNA into the DNA of permissive cells followed by excision of viral and of some host DNA sequences and circularization of these molecules (Lavi and Winocour, 1972; Tai et al,, 1972).
KeywordsLight Band Permissive Cell Equilibrium Centrifugation Selective Hybridization Virus Pool
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