Bidirectional Replication of E. coli DNA: A Physical Demonstration

  • Millicent Masters
  • W. G. McKenna


A number of genetic studies (1–3), some of which have been discussed at this meeting, make it clear that the chromosome of Escherichia coli is replicated bidirectionally. Such studies, however, cannot eliminate the possibility that the single linkage group of E. coli is not in fact composed of two separate structures, each of which is replicated unidirectionally. (One would need to presume, for this to be the case, that these structures associate during Hfr transfer in such a way so as to behave as a single structure.)


Separate Structure Daughter Strand Single Linkage Group Thymine Starvation Drop Fraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Masters, M. and Broda, P. Nature (New Biol.) 232: 137, 1971.Google Scholar
  2. 2.
    Bird, R. E., Lovarn, J., Martuscelli, J. and Caro, L. G. J. Mol. Biol. 70: 549, 1972.CrossRefGoogle Scholar
  3. 3.
    Hohlfield, R. and Vielmetter, W. (in press).Google Scholar
  4. 4.
    Gilbert, W. and Dressier, D. Cold Spring Harbor Symp. Quant. Biol. 33: 473, 1968.PubMedCrossRefGoogle Scholar
  5. 5.
    Watson, J. D. In Molecular Biology of the Gene, p. 291, W. A. Benjamin, Inc. New York, 1970.Google Scholar
  6. 6.
    Prescott, D. M. and Kuempel, P. L. Proc. Nat. Acad. Sci. USA 69: 2842, 1972.PubMedCrossRefGoogle Scholar
  7. 7.
    Weintraub, H. Nature (New Biol.) 236: 145, 1972.Google Scholar
  8. 8.
    Hotz, G. and Walser, R. Photochem. Photobiol. 12: 207, 1970.PubMedCrossRefGoogle Scholar
  9. 9.
    McKenna, W. G. and Masters, M. Nature 240: 539, 1972.CrossRefGoogle Scholar
  10. 10.
    Stein, G. H. and Hanawalt, P. C. J. Mol. Biol. 64: 393, 1972.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Millicent Masters
    • 1
  • W. G. McKenna
    • 1
  1. 1.MRC Molecular Genetics UnitUniversity of EdinburghEdinburghScotland

Personalised recommendations