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RNA and Colicinogenic Factor E1 Replication

  • D. J. Sherratt
  • D. G. Blair
  • D. B. Clewell
  • D. R. Helinski

Abstract

The bacterial non-sex factor plasmid, colicinogenic factor El (ColE1), has a molecular weight of 4.2 × 106 daltons and there are normally about 20 copies present per E. coli cell (Clewell and Helinski, 1972). Unlike sex-factors and the E. coli chromosome, ColE1 continues to replicate in chloramphenicol treated cells for many generations (Bazaral and Helinski, 1970). However addition of rifampicin to chloramphenicol treated cells results in rapid cessation of ColE1 synthesis (Clewell, Evenchick and Cranston, 1972), indicating that RNA synthesis but not protein synthesis is required for ColE1 replication.

Keywords

Sucrose Density Gradient Public Health Service Research Open Circular Form Foundation Research Grant Research Career Development Awardee 
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References

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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • D. J. Sherratt
    • 1
  • D. G. Blair
    • 3
    • 1
  • D. B. Clewell
    • 1
  • D. R. Helinski
    • 2
  1. 1.Department of BiologyUniversity of CaliforniaSan Diego, La JollaUSA
  2. 2.School of BiologyUniversity of SussexFalmer, BrightonEngland
  3. 3.Department of ChemistryU.C.S.D.USA

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