The Role of the Host Membrane in the Morphogenesis of the Head of Coliphage T4

  • Pamela J. Siegel
  • Moselio Schaechter


Head morphogenesis in T4 represents an ideal system for studying the interaction of proteins with nucleic acid since many of the relevant components can be dissected genetically. T4 head morphogenesis has been studied by electron microscopic examination of cells infected with conditional lethal mutants under non-permissive conditions. However, the structures accumulated often are not normal intermediates. In vitro complementation (the formation of a head from its pieces) has not been achieved for T4. In fact, it has been carried out with good efficiency only with Tobacco Mosaic Virus. Here we describe a new approach for the examination of the early events of T4 head morphogenesis which conceivable could be used to study other viruses.


Tobacco Mosaic Virus Host Membrane Major Coat Protein Lauroyl Sarcosinate Wild Type Infection 
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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Pamela J. Siegel
    • 1
  • Moselio Schaechter
    • 1
  1. 1.Department of Molecular Biology and MicrobiologyTufts University School of MedicineUSA

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