Escherichia coli DNA Polymerases II and III
When cells of an E. coli mutant (pol Al−)1 which is deficient in DNA polymerase I (pol I) are lysed in a French pressure cell, they yield a cell-free extract with a small but demonstrable amount of DNA polymerizing activity.2 Following the removal of nucleic acid from such an extract, phosphocellulose chromatography can resolve two separate DNA polymerases.3 Both of these DNA polymerases, called DNA polymerase (pol II) and DNA polymerase III (pol III), have been shown not only to be physically, catalytically, and genetically distinct from each other, but that they are similarly distinct from Pol I.4–6 It will be the subject of this talk to review the work which led to these conclusions.
KeywordsDEAE Cellulose Nuclease Activity Chromosome Replication Approximate Molecular Weight Standard Reaction Mixture
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