Advertisement

Glucose Isomerase Cells Entrapped in Cellulose Acetates

  • M. J. Kolarik
  • B. J. Chen
  • A. H. EmeryJr.
  • H. C. Lim

Summary

Entrapment of whole cells containing glucose isomerase in primary and secondary cellulose acetates was investigated. These enzyme-acetate complexes were formed into fibers and membranes to the extent of one gram of whole cells entrapped in two grams of acetate. With fibers measuring about 250 × 500 microns in cross section the rate of diffusion of substrate into the fiber limits the activity of the resulting cell-acetate complex to the extent of 17% of the original cell activity. On the other hand, with membranes measuring 10 – 20 microns thick the activity was as high as 57% of the original cell activity. However, cells leaked from these membranes. The leakage of cells from other membranes was reduced to about 5% by varying the solvent system. Permeability was also affected by the solvent system used.

Keywords

Solvent System Cellulose Acetate Ethylene Dichloride Feed Concentration Glucose Isomerase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    G.J.H. Melrose. Rev. Pure Appl. Chem., 21, 83 (1971).Google Scholar
  2. 2.
    F. Leuschner, British Patent 953,414 (1964).Google Scholar
  3. 3.
    W.R. Vieth, S.S. Wang, and R. Saini, Biotechnol. Bioeng., 15, 565 (1973).CrossRefGoogle Scholar
  4. 4.
    D. Dinelli, Process Biochem., 7, 9 (1972).Google Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • M. J. Kolarik
    • 1
  • B. J. Chen
    • 1
  • A. H. EmeryJr.
    • 1
  • H. C. Lim
    • 1
  1. 1.School of Chemical EngineeringPurdue UniversityWest LafayetteUSA

Personalised recommendations