Glucose Isomerase Cells Entrapped in Cellulose Acetates
Entrapment of whole cells containing glucose isomerase in primary and secondary cellulose acetates was investigated. These enzyme-acetate complexes were formed into fibers and membranes to the extent of one gram of whole cells entrapped in two grams of acetate. With fibers measuring about 250 × 500 microns in cross section the rate of diffusion of substrate into the fiber limits the activity of the resulting cell-acetate complex to the extent of 17% of the original cell activity. On the other hand, with membranes measuring 10 – 20 microns thick the activity was as high as 57% of the original cell activity. However, cells leaked from these membranes. The leakage of cells from other membranes was reduced to about 5% by varying the solvent system. Permeability was also affected by the solvent system used.
KeywordsSolvent System Cellulose Acetate Ethylene Dichloride Feed Concentration Glucose Isomerase
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