The Uses of Precipitated Nylon as an Enzyme Support: An α-Galactosidase Reactor
Nylon precipitated from formic acid produces a surface into which enzymes readily adsorb. They can be immobilized on the surface by inter-enzyme crosslinking with glutaraldehyde or dimethyladipimidate. Nylon has been deposited onto the surfaces of porous polyethylene disks which provide a very convenient rigid matrix for a small enzyme reactor. On the other hand, the precipitated nylon does not require a support. It can be used to fill large column reactors through which enzyme and crosslinking agent are passed to make the immobilized enzyme in situ. A new, neutral, thermostable α-galactosidase of molecular weight 150,000 has been immobilized by this latter technique for the purpose of hydrolyzing the oligosaccharides in soy milks and beet sugar molasses. In addition thermolysin, papain, glucose isomerase and amyloglucosidase reactors have been prepared and studied.
KeywordsImmobilize Enzyme Crosslinking Agent Back Pressure Reactor Design Glucose Isomerase
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