The Use of Tannic Acid and Phenol-Formaldehyde Resins with Glutaraldehyde to Immobilize Enzymes

  • Alfred C. Olson
  • William L. Stanley


Our investigations with immobilized enzymes have been guided by how they may assist in finding out more about enzymic phenomena and how they could ultimately be utilized in processes and analyses, particularly those related to agriculture and food. In the latter area economic as well as scientific factors play a very important role. A complete evaluation of all of these factors is beyond the scope of this paper. However, we have considered many of these factors in both the choice of the systems for immobilization and the applications we have elected to study. The criteria we used in selecting methods for enzyme immobilization included: successful achievement of stable enzyme immobilization; an acceptable degree of retention of enzyme activity; good pH and temperature characteristics; simplicity of the method; use of impure or inexpensive enzyme preparations; a potential for large volume application; and low cost.


Tannic Acid Immobilize Enzyme Glutaric Acid Hydrolyze Lactose Lactose Solution 
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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Alfred C. Olson
    • 1
  • William L. Stanley
    • 1
  1. 1.Western Regional Research Center, Agricultural Research ServiceU.S. Department of AgricultureBerkeleyUSA

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