Studies with Dark Avoidance and Scotophobin

  • Helene N. Guttman
  • G. Matwyshyn
  • M. Weiler


Stability of scotophobin, 5,11-deamidoscotophobin and N-acetyl 5,11-deamidoscotophobin is compared using micro-dansylation followed by chromatographic analysis.

Methods for the routine testing and analysis of results of dark avoidance experiments are considered. The methods allow analysis of results with animals which vary with respect to reaction initiation time, and duration of reaction.

Using the paradigm of dark avoidance activity analysis followed by dark-avoidance training, it is shown that there is low-grade, variable and transient dark avoidance activity for the following compounds: 5,11-deamidoscotophobin, N-acetyl 5,11-deamidoscotophobin, 8-15 amino acid fragment of deamidoscotophobin, and degraded scotophobin.

Proof is presented that scotophobin passes the blood-brain barrier.

Scotophobin does not exert its action by causing a general reduction of activity of goldfish.

Participation of various amino acid residues of scotophobin in binding and activity is discussed. During manifestation of dark avoidance activity, scotophobin is bound to a large molecular weight cellular site. Synaptic membranes or S-100 proteins are candidates for binding materials.

Theoretical considerations on relationship of induction of behavioral alteration and of other inducible, biological processes are briefly considered.


Passive Transfer Saving Activity Amino Acid Fragment Passive Reaction Vagal Lobe 
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Copyright information

© Plenum Press, New York 1973

Authors and Affiliations

  • Helene N. Guttman
    • 1
  • G. Matwyshyn
    • 1
  • M. Weiler
    • 1
  1. 1.Department of Biological SciencesUniversity of Illinois at Chicago CircleChicagoUSA

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