Studies with Dark Avoidance and Scotophobin
Stability of scotophobin, 5,11-deamidoscotophobin and N-acetyl 5,11-deamidoscotophobin is compared using micro-dansylation followed by chromatographic analysis.
Methods for the routine testing and analysis of results of dark avoidance experiments are considered. The methods allow analysis of results with animals which vary with respect to reaction initiation time, and duration of reaction.
Using the paradigm of dark avoidance activity analysis followed by dark-avoidance training, it is shown that there is low-grade, variable and transient dark avoidance activity for the following compounds: 5,11-deamidoscotophobin, N-acetyl 5,11-deamidoscotophobin, 8-15 amino acid fragment of deamidoscotophobin, and degraded scotophobin.
Proof is presented that scotophobin passes the blood-brain barrier.
Scotophobin does not exert its action by causing a general reduction of activity of goldfish.
Participation of various amino acid residues of scotophobin in binding and activity is discussed. During manifestation of dark avoidance activity, scotophobin is bound to a large molecular weight cellular site. Synaptic membranes or S-100 proteins are candidates for binding materials.
Theoretical considerations on relationship of induction of behavioral alteration and of other inducible, biological processes are briefly considered.
KeywordsPassive Transfer Saving Activity Amino Acid Fragment Passive Reaction Vagal Lobe
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- Desiderio, D. M., G. Ungar, and P. A. White: The use of mass spectrometry in the structural elucidation of scotophobia, a specific behavior-inducing brain peptide. Chem. Commun. 9, 432–433 (1971).Google Scholar
- Golub, A. M., and J. V. Mcconnell: Empirical issues in inter-animal transfer of information. In: Chemical Transfer of Learned Information (E. J. Fjerdingstad, ed.), pp. 1–29, North-Holland, Amsterdam (1971).Google Scholar
- Gray, W. R., and B. S. Hartley: A fluorescent end-group reagent for proteins and peptides. Biochem. J. 89, 59 (1963).Google Scholar
- Guttman, H. N., and L. Gronke: Passive transfer of learned dark and step-down avoidance. Psychon. Sci. 24, 107–109 (1971).Google Scholar
- Guttman, H. N., G. Matwyshyn, and G. H. Warriner III: Synthetic scotophobin-mediated passive transfer of dark avoidance. Nature New Biology 235, 26–27 (1972).Google Scholar
- Katz, M. S., and W. C. Halstead: Protein organization and mental function. Comp. Psych. Monogr. 20, 1–38 (1950).Google Scholar
- Russell, R. W., G. Ungar, and E. Usdin: Seminar on the requirements for testing of hypotheses about molecular coding of experience: transfer studies. Psychopharm. Bull. 8, 5–13 (1972).Google Scholar
- Ungar, G.: Role of proteins and peptides in learning and memory. In: Molecular Mechanisms in Memory and Learning (G. Ungar, ed.), pp. 149–175, Plenum, New York (1970).Google Scholar
- Ungar, G., and S. R. Burzynski: Detection of a behavior-inducing peptide (scotophobin) in brain by ultramicro-analytical method. Fed. Proc. 31, 398 (1972).Google Scholar