On the Application of the Single Photon Counting Technique for the Investigation of Dyes with Short Fluorescence Decay Time
The single photon method is used in fluorescence decay measurements with a excitation flash of small width and fast decay and an analog computer for evaluation of the rate parameters. With this equipment decay times down to 0.2 nanoseconds can be determined accurately and easily. This technique is applied to the investigation of the reaction kinetics in the excited state of 7-hydroxycoumarin and 4-methylumbelliferon in water-ethanol mixtures. Rate parameters for an adapted model and assignments of the fluorescent reaction products are given.
KeywordsDecay Time Analog Computer Fluorescence Decay Flash Lamp Fluorescence Kinetic
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