Dependence of Poly U-Directed Cell-Free System on Ratios of Divalent and Monovalent Cations
An obligatory component of a protein-synthesizing cell-free system are inorganic cations: divalent ones, primarily Mg++, and monovalent ones, NH 4 + or K+ (1). Up to the present, the optimal concentration of these cations for use in cell-free systems was chosen by varying the concentration of one cation on the background of an invariable concentration of the other. However, an absolute optimum for both cations cannot be determined in such a manner, as the concentration of the second cation itself affects the position of the optimum for the first cation. The aim of the present communication is to determine the dependence of the cell-free system activity on the ratios of Mg++ and NH 4 + cations in the medium and to find the optimum of the system for the two denoted factors. A polyU-directed cell-free system of polyphenylalanine synthesis on Escherichia coli ribosomes was taken as the object of the investigation.
KeywordsPyruvate Kinase Enzyme Fraction Obligatory Component Isoactive Line Total tRNA
Unable to display preview. Download preview PDF.
- 1.Spirin, A. S., and Gavrilova, L. P., “The Ribosome,” Springer-Verlag, Berlin-Heidelberg-New York, 1969.Google Scholar
- 2.Spirin, A. S., Sofronova, M. Y., and Sabo, B., Molekularnaya Biologiya (USSR) 4, 618 (1970).Google Scholar
- 6.Sabo, B., and Spirin, A. S., Molekularnaya Biologiya (USSR) 4, 628 (1970).Google Scholar