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Interactions at the Surface of Plant Cell Protoplasts; An Electrophoretic Freeze-etch Study

  • B. W. W. Grout
  • J. H. M. Willison
  • E. C. Cocking

Abstract

The physical limitation of the cell protoplast has been demonstrated both mechanically, and microscopically, as a tangible barrier between cell cytoplasm and the external environment. In higher plant cells the isolation afforded by this limiting membrane is enhanced by the normally cellulosic cell wall, usually very close to the membrane outer surface. The work to be described is an attempt to illustrate aspects of structure and some functions of the outer protoplast membrane, or plasmalemma, and from this information, to discern something of the dynamic nature of this plasma membrane.

Keywords

Zeta Potential Sodium Nitrate Granule Density Fracture Face Cytoplasmic Vesicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    M. A. Mayo and E. C. Cocking, Protoplasma, 68 (1969) 223.CrossRefGoogle Scholar
  2. 2.
    J. H. M. Willison, B. W. W. Grout and E. C. Cocking, J. Bioenergetics, 2 (1972) 371.CrossRefGoogle Scholar
  3. 3.
    E. D. Korn and R. A. Weismann, J. Cell. Biol., 34 (1967) 219.PubMedCrossRefGoogle Scholar
  4. 4.
    T. R. Ricketts, Protoplasma, 73 (1971) 387.CrossRefGoogle Scholar
  5. 5.
    A. C. Allison, P. Davies and S. de Petris, Nature New Biol., 232 (1971) 153.PubMedGoogle Scholar
  6. 6.
    L. A. Withers and E. C. Cocking, J. Cell Sci., 11 (1972) 59.PubMedGoogle Scholar
  7. 7.
    A. C. Allison, in: Symposium on Cell Interactions, Londno 1971, Elsevier, Amsterdam.Google Scholar
  8. 8.
    H. Stanley-Bennett, in: Handbook of Molecular Cytology, 15, A. Lima-de-Faria (ed.), North-Holland, Amsterdam, 1969, p. 1294.Google Scholar
  9. 9.
    J. B. Power, and E. C. Cocking, J. Expt. Bot., 21 (1970) 64.CrossRefGoogle Scholar
  10. 10.
    H. Moor and K. Muhlethaler, J. Cell Biol., 17 (1963) 609.PubMedCrossRefGoogle Scholar
  11. 11.
    G. D. Parfitt, Journal of Oil and Colour Chemists Association, 51 (1968) 137.Google Scholar
  12. 12.
    S. Komogata, Researches Electrotech. Lab. Tokyo, 348 (1933).Google Scholar
  13. 13.
    J. Th. G. Overbeek, Adv. Colloid Sci., 3 (1950) 97.Google Scholar
  14. 14.
    M. Smoluckowski, in: Handbuch der Elektrizität, Vol. 2, B. Graetz (ed.), Leipzig, 1914, p. 333.Google Scholar
  15. 15.
    D. C. Henry, Proc. Roy. Soc. (A), 133 (1931) 106.CrossRefGoogle Scholar
  16. 16.
    D. Branton, Proc. Natn. Acad. Sci. USA, 55 (1968) 1048.CrossRefGoogle Scholar
  17. 17.
    D. Branton and D. Southworth, Exp. Cell Res., 47 (1967) 618.CrossRefGoogle Scholar
  18. 18.
    P. G. Pinto da Silva and D. Branton, J. Cell Biol., 45 (1970) 598.CrossRefGoogle Scholar
  19. 19.
    D. Branton, Ann. Rev. Pl. Physiol., 20 (1969) 209.CrossRefGoogle Scholar
  20. 20.
    D. Branton and D. W. Deamer, Membrane Structure, Pro toplasmatologia II. E. I. Springer, Wien, New York (1972) 41.Google Scholar
  21. 21.
    J. L. Kavanau, Structure and Function in Biological Membranes I and II, Holden-Day, San Francisco, 1965.Google Scholar
  22. 22.
    J. A. Lucy, J. Theoret. Biol., 7 (1964) 360.CrossRefGoogle Scholar
  23. 23.
    Z. A. Staehelin and O. Kiermayer, J. Cell Sci., 7 (1970) 787.PubMedGoogle Scholar
  24. 24.
    J. D. Robertson, in: Handbook of Molecular Cytology, 15, A. Lima-de-FariaGoogle Scholar
  25. 25.
    A. Bischoff and H. Moor, Z. Zellforsch., 81 (1967) 303.PubMedCrossRefGoogle Scholar
  26. 26.
    D. Branton, Exp. Cell Res., 45 (1966) 703.CrossRefGoogle Scholar
  27. 27.
    D. H. Northcote, Proc. Roy. Soc. (B), 173 (1969) 21.CrossRefGoogle Scholar
  28. 28.
    Z. Hejnowicz, Protoplasma, 71 (1970) 343.CrossRefGoogle Scholar
  29. 29.
    R. H. A. Coutts, E. C. Cocking and B. Kassanis, J. gen. Virol. (1972) in press.Google Scholar
  30. 30.
    D. Gungiell, J. Theor. Biol., 17 (1967) 451.CrossRefGoogle Scholar
  31. 31.
    E. J. Ambrose and J. A. Forrester, Symp. Soc. Exp. Biol., 22 (1968) 237.PubMedGoogle Scholar
  32. 32.
    G. Poste and A. C. Allison, J. Theor. Biol., 32 (1971) 165.PubMedCrossRefGoogle Scholar
  33. 33.
    J. A. Lucy, Nature, 227 (1970) 814.CrossRefGoogle Scholar

Copyright information

© Plenum Publishing Company Limited 1972

Authors and Affiliations

  • B. W. W. Grout
    • 1
  • J. H. M. Willison
    • 1
  • E. C. Cocking
    • 1
  1. 1.Department of BotanyUniversity of NottinghamNottinghamUK

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