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Biogenesis of Mitochondria 23. The Biochemical and Genetic Characteristics of Two Different Oligomycin Resistant Mutants of Saccharomyces Cerevisiae Under the Influence of Cytoplasmic Genetic Modification

  • Carolyn H. Mitchell
  • C. L. Bunn
  • H. B. Lukins
  • Anthony W. Linnane

Abstract

Two classes of Saccharomyces cerevisiae mutants resistant to oligomycin, an inhibitor of mitochondrial membrane bound ATPase are described. Biochemical analysis shows that in vitro the mitochondrial ATPase of both types of mutant are sensitive to oligomycin. In vivo sensitivity of the mutants to oligomycin can be demonstrated following anaerobic growth of the cells, which grossly alters the mitochondrial membrane and renders the ATPase of the mutants sensitive to oligomycin. It is concluded that the mutation to oligomycin resistance in both mutant types is phenotypically expressed as a change in the mitochondrial membrane. The intact mitochondrial membrane in the wild type cell is freely permeable to oligomycin, whereas the resistant mutant is impermeable to oligomycin; alteration of the mitochondrial membrane during isolation of the organelle or physiological modification of the membranes of the mitochondria by anaerobic growth renders the membranes permeable.

These mitochondrial membrane mutants differ in their cross-reference patterns and their genetics. One is resistant to oligomycin only, and behaves like previously reported cytoplasmic mutants. The other shows cross-resistance to inhibitors of mitochondrial protein synthesis as well as to oligomycin; although the mutant appears to arise from a single step mutation its genetic properties are complex and show part-nuclear and part-cytoplasmic characteristics. The implications of the observations are discussed.

Keywords

Mitochondrial Membrane Anaerobic Growth Mitochondrial Protein Synthesis Mitochondrial ATPase Cytoplasmic Inheritance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Company Limited 1972

Authors and Affiliations

  • Carolyn H. Mitchell
    • 1
  • C. L. Bunn
    • 1
  • H. B. Lukins
    • 1
  • Anthony W. Linnane
    • 1
  1. 1.Biochemistry DepartmentMonash UniversityClaytonAustralia

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