Disturbances of Myelination in Neonatally Thyroidectomized Rat Brains

  • R. Tsujimura
  • N. Kariyama
  • N. Hatotani


It is well known that thyroid deficiency in the neonatal period results in impaired development of the central nervous system. The effects’ in the rats of neonatal hypothyroidism have been the subject of extensive histological (Hamburgh 1969), behavioural (Eayrs and Levine 1963), electroencephalographic (Hatotani and Timiras 1967) and biochemical (Gell et al. 1967, Gómez and Ramirez de Guglielmone 1967, Balázs et al. 1968) studies. To date, however, little attention has been focussed on the cerebral lipids in the states of thyroid dysfunction. The recent works suggested that the lipid content and its fatty acid patterns in the myelin sheath were different from those in the whole brain, the gray matter and the white matter (O’Brien and Sampson 1965a, b). Despite a few studies (Cuarón et al. 1963, Walravens and Chase 1969), the data on the lipid analyses of isolated brain myelin from the hypothyroid animals are completely lacking. The present work was designed to examine in the rats the influences of neonatally induced hypothyroidism on the myelination in terms of changes in myelin lipid content and in normal fatty acid composition of the individual sphingolipid.


Myelin Sheath Fatty Acid Pattern Myelin Lipid Nervonic Acid Hypothyroid Animal 
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  1. Balázs, R., Kovács, S., Teichgräber, P., Cocks, W. A. and Eayrs, J. T. (1968): Biochemical effects of thyroid deficiency on the developing brain. J. Neurochem.15, 1335–1349.PubMedCrossRefGoogle Scholar
  2. Baumann, N. R., Gacque, C. M., Pollet, S. A. and Harpin, M. L. (1968): Fatty acid and lipid composition of the brain of a myelin deficient mutant, the “Quaking” mouse. European J. Biochem.4, 340–344.CrossRefGoogle Scholar
  3. Citarón, A., Gamble, J., Myant, N. B. and Osorio, C. (1963): The effect of thyroid deficiency on the growth of the brain and on the deposition of brain phospholipids in foetal and new-born rabbits. J. Physiol.168, 613–630.Google Scholar
  4. De Robertis, E., Gerschenfeld, H. M. and Wald, F. J. (1958): Cellular mechanism of myelination in the central nervous system. J. biophys. biochem. Gytol.4, 651–658.CrossRefGoogle Scholar
  5. Eayrs, J. T. and Levine, S. (1963): Influence of thyroidectomy and subsequent replacement therapy upon conditioned avoidance learning in the rat. J. Endocr.25, 505–513.CrossRefGoogle Scholar
  6. Folch, J. and Lees, M. (1951): Proteolipids, a new type of tissue lipoproteins. J. biol. Chem.191, 807–817.PubMedGoogle Scholar
  7. Folch, J., Lees, M. and Sloane-Stanley, G. H. (1957): A simple method for the isolation and purification of total lipids from animal tissues. J. biol. Ghem.226, 497–509.Google Scholar
  8. Galli, C. and Re Cecconi Galli, D. (1968): Cerebroside and sulphatide deficiency in the brain of “Jimpy Mice”, a mutant strain of mice exhibiting neurological symptoms. Nature (Lond.)220, 165–166.CrossRefGoogle Scholar
  9. Gell, S. E., Valcana, T. and Timiras, P. S. (1967): Effect of neonatal hypothyroidism and of thyroxine on L-[14C] leucine incorporation in protein in vivo and the relationship to ionic levels in the developing brain of the rat. Brain Res.4, 143–150.CrossRefGoogle Scholar
  10. Gómez, C. J. and Ramirez de Guglielmone, A. E. (1967): Influence of neonatal thyroidectomy on glucose-amino acids interrelations in developing rat cerebral cortex. J. Neurochem.14, 1119–1128.PubMedCrossRefGoogle Scholar
  11. Hamburgh, M. (1969): In: Current Topics in Developmental Biology. Vol. 4. Ed. by A. A. Moscona and A. Monroy. Academic Press, New York and London. pp. 109–148.CrossRefGoogle Scholar
  12. Hatotani, N. and Timiras, P. S. (1967): Influence of thyroid function on the postnatal development of the transcallosal response in the rat. Neuroendocrinology2, 147–156.CrossRefGoogle Scholar
  13. Måtenson, E. (1966): Neutral glycolipids of human kidney: isolation, identification, and fatty acid composition. Biochim. biophys. Acta (Amst.)166, 296–308.Google Scholar
  14. Nomura, M., Nagai, K., Mori, K. and Tsukada, Y. (1969): Neurochemical studies on the experimental hypothyroidism. Bull. Jap. Neurochem. Soc.8, 56–59. (In Japanese.)Google Scholar
  15. Norton, W. T. and Autilo, L. A. (1965): The chemical composition of bovine CNS myelin. Ann. N. Y. Acad. Sci.122, 77–85.PubMedCrossRefGoogle Scholar
  16. Nussbaum, J. L., Neskovic, N. and Mandel, P. (1969): A study of lipid components in brain of the “Jimpy” mouse, a mutant with myelin deficiency. J. Neurochem.16, 927–934.PubMedCrossRefGoogle Scholar
  17. O’Brien, J. S. (1965): Stability of the myelin membrane. Science147, 1099–1107.PubMedCrossRefGoogle Scholar
  18. O’Brien, J. S. and Sampson, E. L. (1965a): Lipid composition of the normal human brain: gray matter, white matter and myelin. J. Lipid Res.6, 537–544.PubMedGoogle Scholar
  19. O’Brien, J. S. and Sampson, E. L. (1965b): Fatty acid and fatty aldehyde composition of the major brain lipids in normal human gray matter, white matter and myelin. J. Lipid Res.6, 545–551.PubMedGoogle Scholar
  20. Ställberg-Stenhagen, S. and Svennerholm, L. (1965): Fatty acid composition of human brain sphingomyelins: normal variation with age and changes during myelin disorders. J. Lipid Res.6, 146–155.Google Scholar
  21. Tsujimura, R. (1971): Immunological and biochemical studies on myelination in the brain of chick embryo. J. Embryol. exp. Morph. (In press.)Google Scholar
  22. Uyemura, K. (1965): Biochemical studies on subcellular units of guinea-pig brain cortex. Ad. Neurol. Sci.9, 121–124. (In Japanese.)Google Scholar
  23. Walravens, P. and Chase, H. P. (1969): Influence of thyroid on formation of myelin lipids. J. Neurochem.16, 1477–1484.PubMedCrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest, Hungary 1973

Authors and Affiliations

  • R. Tsujimura
    • 1
  • N. Kariyama
    • 1
  • N. Hatotani
    • 1
  1. 1.Department of PsychiatryMie Prefectural University School of MedicineTsuJapan

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