Psychoneuroendocrine Interactions in a Reflex Ovulator

  • J. Hilliard
  • Ch. H. Sawyer


Within the past two years, radioimmuno- and protein-binding assays have provided new information concerning steroid-gonadotrophin levels in cyclic-ovulating mammals. In all species studied so far, the normal preovulatory rise in plasma luteinizing hormone (LH) is preceded by an elevation in urinary or plasma estrogen. Scaramuzzi et al. (1970) obtained evidence to support a causal relationship between high estrogen secretion and LH discharge in the ewe by simultaneous radioimmunoassays of plasma LH and ovarian venous estrogen. In their studies, an elevation in estrogen concentration was measurable 24 hours before the pro-estrus rise in circulating LH. A similar estrogen-LH relationship has been demonstrated in the rat by several investigators (Exley and Dutton 1970, Brown-Grant et al. 1970, Neill et al. 1971).


Luteinizing Hormone Luteinizing Hormone Surge Plasma Progesterone Luteinizing Hormone Discharge Estradiol Benzoate 
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  1. Abraham, G. E. and Klaiber, E. L. (1970): Plasma immunoreactive estrogens and LH during the menstrual cycle. Amer. J. Obstet. Gynec.108, 528.PubMedGoogle Scholar
  2. Abraham, G. E., Odell, W. D., Swerdloff, R. S. and Hopper, K. (1971): Simultaneous radioimmunoassay of plasma progesterone (P), 17-hydroxyprogesterone (17-OH P), Estradiol (E-2), LH and FSH during the menstrual cycle. In: Program of the 53rd Meeting of the Endocrine Society, A-160, Abst. 236.Google Scholar
  3. Adams, C. E. (1958): Egg development in the rabbit: The influence of post-coital ligation of the uterine tube and of ovariectomy. J. Endocr.16, 283.PubMedCrossRefGoogle Scholar
  4. Arimura, A. and Schally, A. V. (1971): Augmentation of pituitary responsiveness to LH-releasing hormone (LH-RH) by estrogen. Proc. Soc. exp. Biol. (N.Y.)136, 290.Google Scholar
  5. Austin, C. R. and Braden, A. W. H. (1954): Time relations and their significance in the ovulation and penetration of eggs in rats and rabbits. Austr. J. biol. Sci.7, 179.Google Scholar
  6. Baird, D. T. and Guevara, A. (1969): Plasma estrogens in non-pregnant women and in men. J. clin. Endocr.29, 149.PubMedCrossRefGoogle Scholar
  7. Blandau, R. J. (1969): Gamete transport —comparative aspects. In: The Mammalian Oviduct. Ed. by E. S. E. Hafez and R. J. Blandau. University of Chicago Press, Chicago — London, p. 164.Google Scholar
  8. Blandau, R. J. and Boling, J. L. (1970): An experimental approach to the study of egg transport through the oviducts of mammals. Conference on the Regulation of Mammalian Reproduction, NIH, Bethesda, Md. USA. (In press).Google Scholar
  9. Bogdanove, E. M., Hilliard, J. and Sawyer, C. H. (1971): Serum LH patterns in the female rabbit as determined by radioimmunoassay. In: Program of the 53rd Meeting of the Endocrine Society. A-76, Abst. 68.Google Scholar
  10. Boling, J. L. and Blandau, R. J. (1971): Egg transport through the ampullae of the oviducts of normal estrous rabbits, rabbits injected with gonadotrophins, progestins and CN-55, 945–27. Biol. Abst. (In press).Google Scholar
  11. Brown-Grant, K., Exley, D. and Naftolin, F. (1970): Peripheral plasma oestradiol and luteinizing hormone concentrations during the oestrous cycle in the rat. J. Endocr.48, 295.PubMedCrossRefGoogle Scholar
  12. Bryans, F. E. (1951): Progesterone of the blood in the menstrual cycle of the monkey. Endocrinology48, 733.PubMedCrossRefGoogle Scholar
  13. Bitrdick, H. O. and Pincus, G. (1935): The effect of oestrin injection upon the developing ova of mice and rabbits. Amer. J. Physiol.111, 201.Google Scholar
  14. Chang, M. C. (1955): Fertilization and normal development of follicular oocytes in the rabbit. Science121, 867.PubMedCrossRefGoogle Scholar
  15. Corker, S. S., Exley, D. and Naftolin, F. (1969): Assay of 17β-oestradiol by competitive protein binding methods. Nature (Lond.)222, 1063.CrossRefGoogle Scholar
  16. Eaton, L. W., Jr. and Hilliard, J. (1971): Estradiol-17β, progesterone and 20α-hydroxypregn-4-en-3-one in rabbit ovarian venous plasma. I. Steroid secretion from paired ovaries with and without corpora lutea; effect of LH. Endocrinology89, 105.PubMedCrossRefGoogle Scholar
  17. Endröczi, E. and Hilliard, J. (1965): Luteinizing hormone releasing activity in different parts of rabbit and dog brain. Endocrinology71, 667.CrossRefGoogle Scholar
  18. Everett, J. W. (1948): Progesterone and estrogen in the experimental control of ovulation time and other features of the estrous cycle in the rat. Endocrinology43, 389.PubMedCrossRefGoogle Scholar
  19. Exley, D. and Dutton, A. (1970): Peripheral plasma 17β-oestradiol concentrations during the estrous cycle of a 4-day cyclic rat. In: 3rd International Congress on Hormonal Steroids, Hamburg. p. 232.Google Scholar
  20. Fee, A. R. and Parkes, A. S. (1929): Studies on ovulation. I. The relation of the anterior pituitary body to ovulation in the rabbit. J. Physiol. (Lond.)67, 383.Google Scholar
  21. Greenwald, G. S. (1961): A study of the transport of ova through the rabbit oviduct. Fertil. and Steril.12, 80.Google Scholar
  22. Harper, N. J. K. (1966): Hormonal control of transport of eggs in cumulus through the ampulla of the rabbit oviduct. Endocrinology78, 568.PubMedCrossRefGoogle Scholar
  23. Hayward, J. N., Hilliard, J. and Sawyer, C. H. (1964): Time of release of pituitary gonadotrophin induced by electrical stimulation of the rabbit brain. Endocrinology74, 108.PubMedCrossRefGoogle Scholar
  24. Hilliard, J. and Sawyer, C. H. (1964): Synthesis and release of progestin by rabbit ovary in vivo. In: Proceedings Ist International Congress on Hormonal Steroids. Vol. 1. Academic Press, New York. p. 263.Google Scholar
  25. Hilliard, J. and Eaton, L. W., Jr. (1971): Estradiol-17β, progesterone, and 20α-hydroxypregn-4-en-3-one in rabbit ovarian venous plasma. II. From mating through implantation. Endocrinology (In press).Google Scholar
  26. Hilliard, J., Endröczi, E. and Sawyer, C. H. (1961): Stimulation of progestin release from rabbit ovary in vivo. Proc. Sgc. exp. Biol. (N.Y.)108, 154.Google Scholar
  27. Hilliard, J., Hayward, J. N. and Sawyer, C. H. (1964): Postcoital patterns of secretion of pituitary gonadotropin and ovarian progestin in the rabbit. Endocrinology75, 957.PubMedCrossRefGoogle Scholar
  28. Hilliard, J., Penardi, R. and Sawyer, C. H. (1967): A functional role for 20α-hydroxypregn-4-en-3-one in the rabbit. Endocrinology80, 901.PubMedCrossRefGoogle Scholar
  29. Hilliard, J., Spies, H. G., Lucas, L. and Sawyer, C. H. (1968a): Effect of prolactin on progestin release and cholesterol storage by rabbit ovarian interstitium. Endocrinology82, 122.PubMedCrossRefGoogle Scholar
  30. Hilliard, J., Spies, H. G. and Sawyer, C. H. (1968b): Cholesterol storage and progestin secretion during pregnancy and pseudopregnancy in the rabbit. Endocrinology82, 157.PubMedCrossRefGoogle Scholar
  31. Hilliard, J., Schally, A. V. and Sawyer, C. H. (1971): Progesterone blockade of the ovulatory response to intrapituitary infusion of LH-RH in rabbits. Endocrinology88, 730.PubMedCrossRefGoogle Scholar
  32. Kawakami, K. and Sawyer, C. H. (1959): Neuroendocrine correlates of changes in brain activity thresholds by sex steroids and pituitary hormones. Endocrinology65, 652.PubMedCrossRefGoogle Scholar
  33. Kawakami, M., Terasawa, E., Ibuki, T. and Manaka, M. (1971): Effects of sex hormones and ovulation-blocking steroids and drugs on electrical activity of the rat brain. In: Steroid Hormones and Brain Function. Ed. C. H. Sawyer and R. A. Gorski. University of California Press.Google Scholar
  34. Korenman, S. G., Perrin, L. E. and McCallum, T. P. (1969): A radioligand binding assay system for estradiol measurements in human plasma. J. clin. Endocr.29, 879.PubMedCrossRefGoogle Scholar
  35. Krey, L. C. and Everett, J. W. (1971): Ovulation after estrogen treatment early in the rat estrous cvcle: significance of induced progesterone secretion. Fed. Proc.30, 310. Abst. 658.Google Scholar
  36. Lawton, I. (1970): Effect of ovarian and adrenal hormones in cyclic LH release. Physiologist13, 264.Google Scholar
  37. Linkie, D. M. and Niswender, G. D. (1971): Serum gonadotrophins during pregnancy in the rat. Fed. Proc.30, 535.Google Scholar
  38. McLaren, A. (1969): Mechanisms affecting embryo development. In: The Mammalian Oviduct. Ed. by E. S. E. Hafez and R. J. Blandau. University of Chicago Press, Chicago — London. p. 447.Google Scholar
  39. Neill, J. D., Freeman, M. E. and Tillson, S. A. (1971): Control of the proestrous “surge” of prolactin and luteinizing hormone secretion by estrogens in the rat. Fed. Proc.30, 474.Google Scholar
  40. Resko, J. A. (1969): Endocrine control of adrenal progesterone secretion in the ovariectomized rat. Science164, 70.PubMedCrossRefGoogle Scholar
  41. Rondell, P. (1970): Follicular processes in ovulation. Fed. Proc.29, 1875.PubMedGoogle Scholar
  42. Ross, G. T., Cargille, C. M., Lipsett, M. B., Rayford, P. L., Marshall, J. T., Strott, C. A. and Rodbard, D. (1970): Pituitary and gonadal steroids in women. Recent Progr. Hormone Res.26, 1.PubMedGoogle Scholar
  43. Sawyer, C. H. (1970): Electrophysiological correlates of release of pituitary ovulating hormone. Fed. Proc.29, 1895.PubMedGoogle Scholar
  44. Sawyer, C. H. and Markee, J. E. (1959): Oestrogen facilitation of release of pituitary ovulating hormone in the rabbit in response to vaginal stimulation. Endocrinology65, 614.PubMedCrossRefGoogle Scholar
  45. Scaramuzzi, R. J., Caldwell, B. V. and Moor, R. M. (1970): Radioimmunoassay of LH and estrogen during the estrous cycle in the ewe. Biol. Beprod.3, 110.Google Scholar
  46. Smith, P. E. and White, W. E. (1931): The effect of hypophysectomy on ovulation and corpus luteum formation in the rabbit. J. Amer. med. Ass.97, 1861.CrossRefGoogle Scholar
  47. Westman, A. (1926): A contribution to the question of the transit of the ovum from ovary to uterus in rabbits. Acta obstet. gynec. scand.5, 1.CrossRefGoogle Scholar
  48. Westman, A. and Jacobsohn, D. (1937): Über Oestrinwirkungen auf die Corpus luteum-Function. Acta obstet. gynec. scand.17, 13.CrossRefGoogle Scholar
  49. Young, W. C. (1961): The mammalian ovary. In: Sex and Internal Secretions. Vol. I. Ed. by W. C. Young. Williams and Wilkins, Baltimore. p. 449.Google Scholar

Copyright information

© Akadémiai Kiadó, Budapest, Hungary 1973

Authors and Affiliations

  • J. Hilliard
    • 1
    • 2
  • Ch. H. Sawyer
    • 1
    • 2
  1. 1.Medical Research ProgramsLong Beach VA HospitalLong BeachUSA
  2. 2.Department of AnatomyUCLA School of MedicineLos AngelesUSA

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