Methods of Studying the Primary Structure of Transfer RNA
Theoretical aspects of the study of the primary structure of nucleic acids were widely discussed early in the 1960s (Habermann, 1963; Tumanyan and Kiselev, 1963; Rice and Bock, 1963; Mirzabekov and Baev, 1965) and initially some workers were doubtful that the nucleotide sequence could ever be determined in a given nucleic acid. However, along with these theoretical investigations, methods of analysis of primary structure were intensively developed and improved, and considerable progress has now been achieved in this field. By the use of various methods of chromatography and ofcoun-tercurrent distribution highly purified specimens of tRNA, suitable for structural research, have been isolated. Great advances have been made in the fields of purification of nucleases and the study of their specificity, with the result that these enzymes have been converted into a fundamental tool for the study of nucleic-acid structure. Parallel developments have taken place in methods of fractionation, identification, and study of the structure of the nucleic-acid fragments. The results obtained by these methods have put an end to the theoretical disputes, because the determination of the primary structure of tRNA has been shown to be a practical possibility.
KeywordsPartial Hydrolysis Phosphodiester Bond Micrococcal Nuclease tRNA Molecule Terminal Nucleotide
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