Equipment for Column Chromatography

  • S. G. Perry
  • R. Amos
  • P. I. Brewer


Until comparatively recently equipment for liquid chromatography was relatively simple and unsophisticated. Apart from glassware one might have used a vibrator to consolidate the bed of stationary phase in the column, perhaps have used a syringe to add sample to the head of the column, and, at the column exit, have collected effluent with a fraction collector. Today the picture is very different and, at the time of writing (late 1970), completely integrated commercial liquid chromatographs are available and cost $2-20M, reflecting the cost of the various newly evolved and often complex components which make up a modern high-performance liquid chromatog raphy system.


Column Chromatography Dead Volume Rotary Pump Photometric Detector Differential Refractometer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    1969–1970 Laboratory guide, Anal. Chem. July 1969.Google Scholar
  2. 2.
    International chromatography guide, J. Chromatog. Sci. ,May 1969.Google Scholar
  3. 3.
    Johnson, J. F., and Cantow, M. R., J. Chromatog. 28, 128 (1967).CrossRefGoogle Scholar
  4. 4.
    Snyder, L. R., and Saunders, D. L., J. Chromatog. Sci. 7, 195 (1969).Google Scholar
  5. 5.
    Rosett, T., J. Chromatog. 18, 498 (1965).CrossRefGoogle Scholar
  6. 6.
    Middleton, W. R., Anal. Chem. 39, 1839 (1967).CrossRefGoogle Scholar
  7. 7.
    Hurrell, R. A., and Amos, R., in Gas Chromatography 1962 ,van Swaay, M., ed., Butterworths, London p. 162, (1963).Google Scholar
  8. 8.
    Scott, R. P. W., Blackburn, D. W. J., and Wilkins, T., J. Gas Chromatog. 5,182 (1967).Google Scholar
  9. 9.
    Stewart, H. N. M. S., Amos, R., and Perry, S. G., J. Chromatog. 38, 209 (1968).CrossRefGoogle Scholar
  10. 10.
    Huber, J. F. K., personal communication.Google Scholar
  11. 11.
    Halasz, I., and Walking, P., J. Chromatog. Sci. 7, 129 (1969).Google Scholar
  12. 12.
    Maggs, R. J., and Young, T. E., in Gas Chromatography 1968 ,Harbourn, C. L. A., ed., Institute of Petroleum, London p. 217, (1969).Google Scholar
  13. 13.
    Snyder, L. R., J. Chromatog. Sci. 7, 352 (1969).Google Scholar
  14. 14.
    Giddings, J. C., Dynamics of Chromatography ,Part I, Marcel Dekker, New York, p. 281, (1965).Google Scholar
  15. 15.
    Piel, E. V., Anal. Chem. 38, 670 (1966).CrossRefGoogle Scholar
  16. 16.
    Snyder, L. R., Anal. Chem. 39, 698 (1967).CrossRefGoogle Scholar
  17. 17.
    Huber, J. F. K., J. Chromatog. Sci. 7, 172 (1969).Google Scholar
  18. 18.
    Billmeyer, F. W., Jr., and Kelley, R. N., J. Chromatog. 34, 322 (1968).CrossRefGoogle Scholar
  19. 19.
    Bombaugh, K. J., King, R. N., and Cohen, A. J., J. Chromatog. 43, 332 (1969).CrossRefGoogle Scholar
  20. 20.
    Smuts, T. W., van Niekerk, F. A., and Pretorius, V., J. Chromatog. Sci. ,7, 127 (1969).Google Scholar
  21. 21.
    Kirkland, J. J., Anal. Chem. 40, 391 (1968).CrossRefGoogle Scholar
  22. 22.
    Stahl, E. (ed.), Thin Layer ChromatographyEnglish Springer-Verlag, Berlin, (1969).Google Scholar
  23. 23.
    Littlewood, A. B., Gas ChromatographyAcademic Press, London and New York (1970).Google Scholar
  24. 24.
    Martin, A. J. P., and Scott, R. P. W., Las Vegas Symposium, 1969.Google Scholar
  25. 25.
    Scott, R. P. W., in Gas Chromatography in Biology and Medicine ,Porter, R., ed., Churchill, London, pp. 54–56, (1969).Google Scholar
  26. 26.
    James, A. T., and Piper, E. A., J. Chromatog. 5, 265 (1961).CrossRefGoogle Scholar
  27. 27.
    Pecsok, R. L., and Saunders, D. L., Anal. Chem. 40, 1756 (1968).CrossRefGoogle Scholar
  28. 28.
    Knudson, G., Ramaley, L., and Holcombe, W. A., Chem. Instr. 1, 325 (1969).CrossRefGoogle Scholar
  29. 29.
    Taylor, A. F., Analyst 88, 145 (1963).CrossRefGoogle Scholar
  30. 30.
    Bright, A. W., and Makin, B., J. Mat. Sci. ,2, 184 (1967).CrossRefGoogle Scholar
  31. 31.
    Kemula, A., Roczniki Chem. 26, 281 (1952).Google Scholar
  32. 32.
    Kemula, A., J. Anal. Chem. (USSR) 22, 562 (1967).Google Scholar
  33. 33.
    Kemula, W., and Witwicki, J., Roczhiki Chem. 29, 1153 (1955).Google Scholar
  34. 34.
    Kemula, W., and Stachurski, Z., Roczhiki Chem. 30, 1285 (1956).Google Scholar
  35. 35.
    Kemula, W., Butkiewicz, D., and Sybitska, D., Modern Aspects of Polarography ,Plenum Press, New York p. 36, (1966).Google Scholar
  36. 36.
    Kemula, W., and Sybitska, D., Anal. Chim. Acta 38, 97 (1967).CrossRefGoogle Scholar
  37. 37.
    Koen, J. G., Huber, J. F. K., Poppe, H., and den Boef, G., J. Chromatog. Sci. 8, 192 (1970).Google Scholar
  38. 38.
    Crompton, T. R., and Cope, B., Anal. Chem. 40, 274 (1968).CrossRefGoogle Scholar
  39. 39.
    Physics Exhbition, London 1967, Chemical Inspectorate of the Ministry of Defence exhibit.Google Scholar
  40. 40.
    Sternberg, J. C., and Carson, L. M., J. Chromatog. 2, 53 (1959).CrossRefGoogle Scholar
  41. 41.
    Simon, W., Clerc, J. T., and Dohner, R. E., Helv. Chim. Acta 48,1628 (1965).CrossRefGoogle Scholar
  42. 42.
    Simon, W., Clerc, J. T., and Dohner, R. E., Microchim. J. 10, 495 (1966).CrossRefGoogle Scholar
  43. 43.
    Poulsen, R. E., and Jensen, H. B., Anal. Chem. ,40, 1206 (1968).CrossRefGoogle Scholar

Further Reading

  1. Conlon, R. D., Liquid chromatography detectors, Anal. Chem. 41 (4), 107A (1969).CrossRefGoogle Scholar
  2. Halasz, I., and Walkling, P., Different types of packed columns in liquid-solid chromato graphy, J. Chromatog. Sci. 7, 129 (1969).Google Scholar
  3. Horvath, C., and Lipsky, S. R., Rapid analysis of ribonucleosides the picomoleGoogle Scholar
  4. level using pellicular cation exchange resin in narrowbore columns, Anal. Chem. 41, 1227 (1969).Google Scholar
  5. Horvath, C., and Lipsky, S. R., Column design in high-pressure liquid chromatography, J. Chromatog. Sci. 7, 109 (1969).Google Scholar
  6. Huber, J. F. K., High-efficiency, high-speed liquid chromatography in columns, J. Chrom atog. Sci. 7, 85 (1969).Google Scholar
  7. Hupe, K. P., and Bayer, E., A microadsorption detector for general use in liquid chromatography, J. Gas Chromatog. 5, 197 (1967).Google Scholar
  8. Jentoft, R. E., and Gouw, T. H., A high-resolution liquid chromatography, Anal. Chem. 40, 1787 (1969).CrossRefGoogle Scholar
  9. Maggs, R. J., Application of a F.I.D. as a monitor for liquid chromatography columns, Column (Pye Unicam) 2 (2,d) (1967).Google Scholar
  10. Veening, H., J. Chem. Ed. 47, (1970). The issues for September, October, and November contain a review of detectors for LC. The papers and reviews listed above typify the recent work in the field of high-speed, high-resolution liquid chromatography which has led to to the revolution in liquid Chromatographic technique.Google Scholar

Copyright information

© Plenum Press, New York 1972

Authors and Affiliations

  • S. G. Perry
    • 1
  • R. Amos
    • 1
  • P. I. Brewer
    • 1
  1. 1.Esso Research CentreEsso Petroleum Company, LimitedAbingdon, BerkshireEngland

Personalised recommendations