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The Technique of Thin-Layer Chromatography

  • S. G. Perry
  • R. Amos
  • P. I. Brewer

Abstract

The discovery of thin-layer chromatography (TLC) is attributed to Izmailov and Shraiber(1), who, in 1938, separated plant extracts by spotting them onto adsorbent layers produced by coating microscope slides with a slurry of the adsorbent with water and allowing to dry. Separation of the mixture into concentric zones was achieved by applying drops of certain solvents to the center of the spot.

Keywords

Cover Plate Solvent Front Phosphomolybdic Acid Precoated Plate Development Chamber 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Further Reading

  1. Stahl, E. (ed.), Dünnschicht-Chromatographic ,Springer-Verlag, Berlin-Heidelberg-New York, 2nd. (1967) (English edition, 1969); Kirchner, J. G., Thin-layer chromatog raphy, in Technique of Organic Chemistry ,Vol. XII, Perry, E. S., and Weissberger, A., eds., Wiley-Interscience, New York (1967). (These two books contain major reviews of the techniques and applications of TLC up until 1966.)Google Scholar
  2. Shellard, E. J., (ed.), Quantitative Paper and Thin Layer Chromatography ,Academic Press, London and New York (1968). (The proceeds of a symposium which reviewed recent developments in various quantitative techniques in paper and thin-layer chromatography.)Google Scholar
  3. Snyder, L. R., RF values in thin-layer chromatography on alumina and silica, in Advan. Chromatog. 4, 3 )1967). (Presents a general theory, which is verified using literature data, for the correlation and prediction of RF values in TLC. International Symposium on Reproducibility in Paper and Thin Layer Chromatography, J. Chromatog. 32, 1–409 (1968). (Original papers and reviews are presented which discuss factors affecting RF values and various quantitative methods of analysis.)Google Scholar

Copyright information

© Plenum Press, New York 1972

Authors and Affiliations

  • S. G. Perry
    • 1
  • R. Amos
    • 1
  • P. I. Brewer
    • 1
  1. 1.Esso Research CentreEsso Petroleum Company, LimitedAbingdon, BerkshireEngland

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