Band Spreading in Chromatography

  • S. G. Perry
  • R. Amos
  • P. I. Brewer


Many outsiders with a scientific training looking in at the practice of chromatography must regard the exercise as an art or mystique, rather than as a science. There is no doubt that empiricism and experience frequently provide the dominating guidelines in setting up an experiment; theoretical considerations, even of a rudimentary nature, only infrequently play a major part in the design and operation of a Chromatographic separation. This situation is even more pronounced in liquid chromatography than in the newer, but enormously more successfully applied technique of gas chromatography. Indeed, despite its seventy or eighty years of history, liquid chromatography has made very little progress, the materials and equipment in use today (at least for most adsorption work in columns) differing rather little from that of Day and Tswett.


Mobile Phase Stationary Phase Molecular Diffusion Solute Molecule Eddy Diffusion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Giddings, J. C., Dynamics of Chromatography ,Part I, Marcel Dekker, New York, 1965.Google Scholar
  2. 2.
    Wilson, J. N., J. Am. Chem. Soc. 62,1583 (1940).CrossRefGoogle Scholar
  3. 3.
    Martin, A. J. P., and Synge, R. M., Biochem. J. 35, 1358 (1941).Google Scholar
  4. 4.
    Van Deemter, J. J., Zuiderweg, F. J., and Klinkenberg, A., Chem. Eng. Sei. 5, 271 (1956).CrossRefGoogle Scholar
  5. 5.
    Ref. 1, pp. 242–243.Google Scholar
  6. 6.
    Wilke, C. R., and Chang, P., Am. Inst. Chem. Eng. J. 1, 264 (1955).CrossRefGoogle Scholar
  7. 7.
    Ref. 1, p. 35.Google Scholar
  8. 8.
    Frenkel, J., Kinetic Theory of Liquids ,Oxford Univ. Press Chapter 4, (1946).Google Scholar
  9. 9.
    Kelley, R. N., and Billmeyer, F. W., Jr., Anal Chem. 41, 874 (1969).CrossRefGoogle Scholar
  10. 10.
    Stewart, H. N. M., Amos, R., and Perry, S. G., J. Chromatog. 38, 209 (1968).CrossRefGoogle Scholar
  11. 11.
    Maggs, R. J., and Young, T. E., Gas Chromatography 1968Harbourn, C. L. A., ed., Inst. Petroleum, London (1969).Google Scholar

Further Reading

  1. Knox, J. H., and Saleem, H., J. Chromatog. Sci. 7, 614 (1969). (Discusses kinetic conditions for optimum speed and resolution in column chromatography.)Google Scholar
  2. Smuts, T. W., van Niekerk, F. A., and Pretorius, V., J. Gas Chromatog. 5,190 (1967). (Discusses the effect of operating parameters on speed of liquid chromatography.)Google Scholar
  3. Huber, J. F. K.,Physical Separation Methods ,Vol. IIB, Wilson, C. L., and Wilson, D. W., Elsevier, Amsterdam Chapter 1, (1968). (Contains a presentation of the theory of liquid chromatography in columns.)Google Scholar

Copyright information

© Plenum Press, New York 1972

Authors and Affiliations

  • S. G. Perry
    • 1
  • R. Amos
    • 1
  • P. I. Brewer
    • 1
  1. 1.Esso Research CentreEsso Petroleum Company, LimitedAbingdon, BerkshireEngland

Personalised recommendations