Towards Chemical Control of Gene Expression: Copper (II) and Thiols, Including Glutathione, are Powerful Reagents for Cleavage of DNA
Sequence-specific cleavage of DNA has many applications in molecular biology, but is limited by the specificities and accessibility of natural restriction endonucleases1. One approach to overcoming this has been to chemically modify a DNA-recognising molecule with a reagent capable of chemical cleavage of DNA. The most commonly used reagent for such chemical cleavage is probably the EDTA:Fe (II) system (1) which has been attached to oligonucleotides1,2, intercalators3, and to a combination of these binding-species4. Minor-groove directed drugs5 and antisense oligodeoxyribonucleoside methylphosphonates6 have joined the catalogue. The other commonly used chemical cleavage systems include bis (1,10- phenanthroline) :Cu (I) (2)7–9 porphyrin metal complexes (3)10–13, and rhodium complexes14. Photochemical cleaving systems for DNA have also been described15–19.
KeywordsHydrogen Peroxide Hydroxyl EDTA Glutathione Sodium Chloride
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