Summary
Four different general categories of mutants defective in cellular response to UV, X-rays, cross-linking and alkylat-ing agents were isolated from Chinese hamster cell lines. Novel types of mutants are described. A new 7th complementation group in the class of UV-sensitive mutants was found. Amongst two complementation groups of X-ray-sensitive mutants, one group resembles Ataxia-telangiectasia cells. Three complementation groups were identified amongst mutants sensitive to cross-linking agents; one mutant (V-H4) is homologous to Fanconi’s anemia.
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References
Collins, A. and R.T. Johnson, 1987, DNA repair mutants in higher eukaryotes, J. Cell Sci. Suppl., 6:61.
Duckworth-Rysiecki, G., Cornish, K., Clarke, C.A., and Buchwald, M., 1985, Identification of two complementation groups in Fanconi anemia, Somatic Cell and Molec. Genet., 11:35.
Fischer, E., Keijzer, W., Thielmann, H.W., Popanda, O., Bohnert, E., Edler, L., Jung, E.G., and Bootsma, D., 1985, A ninth complementation group in Xeroderma pigmen-tosum, Mutation Res., 145:217.
Friedberg, E.C., 1985, DNA repair, Freeman and Co., New York.
Friedberg, E.C., 1987, The molecular biology of nucleotide excision repair of DNA: Recent progress, J. Cell Sci. Suppl., 6:1.
Fuller, L.F., and Painter, R.B., 1988, A Chinese hamster ovary cell line hypersensitive to ionizing radiation and deficient in repair replication, Mutation Res., 193:109.
Hickson, I.D., and Harris, L., 1988, Mammalian DNA repair — use of mutants hypersensitive to cytotoxic agents, Trendsin Genetics, 4:101.
Hoy, C.A., Thompson, L.H., Mooney, C.L., and Salazar, E.P., 1985, Defective DNA cross-link removal in Chinese hamster cell mutants hypersensitive to bifunctional alkylating agents, Cancer Res., 45:1737.
Jaspers, N.G.J., and Bootsma, D., 1982, Genetic heterogeneity in AT studied by cell fusion, Proc. Natl. Acad. Sci. USA, 79:2641.
Jeggo, P.A., and Kemp, L.M., 1983, X-ray-sensitive mutants of Chinese hamster ovary cell line. Isolation and cross-sensitivity to other DNA-damaging agents, Mutation Res., 112:313.
Jeggo, P.A., 1985, Genetic analysis of X-ray-sensitive mutants of the CHO cell line, Mutation Res., 146:265.
Jeggo, P.A., and Holliday, R., 1986, Azacytidine-induced reactivation of a DNA repair gene in Chinese hamster ovary cells, Mol, and Cellular Biol., 6:2944.
Jones, N.J., Cox, R., and Thacker, J., 1987, Isolation and cross-sensitivity of X-ray-sensitive mutants of V79-4 hamster cells, Mutation Res., 183:279.
Kaina, B., 1987, Correction of alkylation hypersensitivity of CHO-W27-1 cells by transfection with human DNA, Car-cinocrenesis, 8:1935.
Meuth, M., 1983, Deoxycytidine kinase-deficient mutant of Chinese hamster ovary cells are hypersensitive to DNA alkylating agents, Mutation Res., 110:383.
Murnane, J.P., and Painter, R.B., 1982, Complementation of the defects in DNA synthesis in irradiated and unirradiated AT cells, Proc. Natl. Acad. Sci. USA, 79:1960.
Robson, C.N., and Hickson, I.D., 1986, Genetic analysis of mitomycin C-sensitive mutant of a Chinese hamster ovary cell line, Mutation Res., 163:201.
Robson, C.N., and Hickson, I.D., 1987, Isolation of alkylating agents-sensitive Chinese hamster ovary cell, Carcinocren-esis, 8:601.
Rubin, J.S., Joyner, A.L., Bernstein, A., and Whitmore, G.F., 1983, Molecular identification of a human DNA repair gene following DNA-mediated gene transfer, Nature, 306:206.
Rubin, J.S., Prideaux, V.R., Willard, H.F., Dulhanty, A.M., Whitmore, G.F., and Bernstein, A., 1985, Molecular cloning and chromosomal localization of DNA sequences associated with human DNA repair gene, Molec. Cell.Biol., 5:398.
Siciliano, M.J., Stallings, R.L., Adair, G.M., Humphrey, R.M., and Siciliano, J., 1983, Provisional assignment of TPI, GPI, and PEPD to Chinese hamster autosomes 8 and 9: a cytogenetic basis for functional haploidy of an autosomal linkage group in CHO cells, Cytoqenet. Cell Genet., 35:15.
Siminovitch, L., 1976, On the nature of hereditable variations in culture somatic cells, Cell, 7:1.
Sinha, B.K., Trush, M.A., Kennedy, K.A., and Mimnaugh, E.G., 1984, Enzymatic activation and binding of adriamycin to nuclear DNA, Cancer Res., 44:2892.
Stefanini, M., Keijzer, W., Westerveld, A., and Bootsma, D., 1985, Interspecies complementation analysis of xeroderma pigmentosum and UV-sensitive Chinese hamster cells, Exp.Cell Res., 161:373.
Tewey, K.M., Chen, G.L., Nelson, E.M., and Liu, L.F., 1984, Intercalative antitumor drugs interfere with the breakage-reunion reaction of mammalian DNA with topoisomerase II, J. Biol. Chem., 259:9182.
Thompson, L.H., Busch, D.B., Brookman, K., Mooney, C.L., and Glaser, D.A., 1981, Genetic diversity of UV-sensitive DNA repair mutants of Chinese hamster ovary cells, Proc. Nat.Acad. Sci., U.S.A., 78:3734.
Thompson, L.H., and Carrano, A.V., 1983, Analysis of mammalian cell mutagenesis and DNA repair using in vitro selected CHO cell mutants. In: Cellular responses to DNA damage, UCLA Symposia on Molecular and Cellular Biology, New Series, Vol. 11 (ed. E.C. Friedberg and B.A. Bridges), pp 125–143, New York, Alan R. Liss.
Thompson, L.H., Mooney, C.L., and Brookman, K.W., 1985, Genetic complementation between UV-sensitive CHO mutants and xeroderma pigmentosum fibroblasts, Mutation Res., 150:423.
Thompson, L.H., Salazar, E.P., Brookman, K.W., Collins, C.C., Stewart, S.A., Busch, D.B., and Weber, C.A., 1987, Recent progress with the DNA repair mutants of Chinese hamster ovary cells. In: The Molecular Biology of DNA Repair, J.Cell Sci. Suppl., 6:97.
Weber, C.A., Salazar, E.P., Stewart, S.A., and Thompson, L.H., 1988, Molecular cloning and biological characterization of a human gene, ERCC2, that corrects the nucleotide excision repair defect in CHO UV5 cells, Mol. Cell.Biol., 8:1137.
Westerveld, A., Hoeijmakers, J.H.J., Duin, M. van, Wit, J. de, Odijk, H., Pastink, A., Wood, R.D., and Bootsma, D., 1984, Molecular cloning of a human DNA repair gene, Nature, 310:425.
Wood, R.D., and Burki, H.J., 1982, Repair capability and the cellular age response for killing and mutation induction after UV, Mutation Res., 95:505.
Zdzienicka, M.Z., and Simons, J.W.I.M., 1986, Analysis of repair processes by the determination of the induction of cell killing and mutations in two repair-deficient Chinese hamster ovary cell lines, Mutation Res., 166:59.
Zdzienicka, M.Z., and Simons, J.W.I.M., 1987, Mutagen-sensi-tive lines are obtained with a high frequency in V79 Chinese hamster cells, Mutation Res., 178:235.
Zdzienicka, M.Z., Roza, L., Westerveld, A., Bootsma, D., and Simons, J.W.I.M., 1987, Biological and biochemical consequences of the human ERCC1 repair gene after transfection into a repair-deficient CHO cell line, Mutation Res., 183:69.
Zdzienicka, M.Z., Schans, G.P. van der, Westerveld, A., Zeeland, A.A. van, and Simons, J.W.I.M., 1988a, Pheno-typic heterogeneity within the first complementation group of UV-sensitive mutants of Chinese hamster cell lines, Mutation Res., 193:31.
Zdzienicka, M.Z., Schans, G.P. van der, and Simons, J.W.I.M., 1988b, Identification of a new seventh complementation group of UV-sensitive mutants in Chinese hamster cells, Mutation Res., 194:165.
Zdzienicka, M.Z., Tran, Q., Schans, G.P. van der, and Simons, J.W.I.M., 1988c, Characterization of an X-ray sensitive mutant of V79 Chinese hamster cells, Mutation Res., 194:239.
Zdzienicka, M.Z., Jaspers, N.G.J., Schans, G.P. van der, Natarajan, A.T., and Simons, J.W.I.M., 1989. Ataxia-telangiectasia-like Chinese hamster V79 cell mutants with radioresistant DNA synthesis, chromosomal instability, and normal DNA strand break repair, Cancer Res., 49:1481–1485.
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© 1989 Plenum Press, New York
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Zdzienicka, M.Z., Simons, J.W.I.M., Lohman, P.H.M. (1989). Chinese Hamster Cell Lines Defective in DNA Repair. In: Lambert, M.W., Laval, J. (eds) DNA Repair Mechanisms and Their Biological Implications in Mammalian Cells. NATO ASI Series, vol 182. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1327-4_43
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DOI: https://doi.org/10.1007/978-1-4684-1327-4_43
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