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Glycerol-3-Phosphate Acyltransferase from Squash Chloroplasts: Protein Characterization by Purification and cDNA Cloning

  • I. Nishida
  • O. Ishizaki
  • N. Murata

Abstract

Acyl-(acyl-carrier protein):glycero1-3-phosphate acyltransferase (glycero-P acyltransferase) in higher-plant chloroplasts transfers the acyl group from acyl-(acy 1-carrier protein) to the C-1 position of glycerol 3-phosphate to synthesize 1-acylglycerol 3-phosphate. Since this reaction is the first step of glycerolipid synthesis in the chloroplasts, it is of special interest to study this enzyme.

Keywords

Isomeric Form Relative Molecular Mass Acyl Transferase Protein Characterization Glycerolipid Synthesis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Hopp, T. P. and Woods, K. R. (1981). Prediction of protein antigenic determinants from amino acid sequences. Proc. Natl, Acad. Sci. USA 78, 3824–3828.CrossRefGoogle Scholar
  2. Ishizaki, O., Nishida, I., Agata, K., Eguchi, G. and Murata, N. (1988). Cloning and sequence determination of a cDNA for plastid glycerol-3- phosphate acyltransferase from squash. In this book.Google Scholar
  3. Lightner, V. A., Bell, R. M. and Modrich, P. (1983). The DNA sequences encoding plsB and dgk loci of Escherichia coli. J. Biol. Chem. 258, 10856–10861.PubMedGoogle Scholar
  4. Nishida, I., Frentzen, M., Ishizaki, O. and Murata, N. (1987). Purification of isomeric forms of acyl-[acyl-carrier-protein]:glycerol-3- phosphate acyltransferase from greening squash cotyledons. Plant Cell Physiol. 28, 1071–1079.Google Scholar

Copyright information

© Akadémiai Kiadó, Budapest 1989

Authors and Affiliations

  • I. Nishida
    • 1
  • O. Ishizaki
    • 1
  • N. Murata
    • 1
  1. 1.National Institute for Basic BiologyMyodaiji, OkazakiJapan

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