Hepatic Processing of Insulin: Characterization and Modulation
The liver is a major target for insulin, up to 50% of the circulating insulin being taken up after a single pass. Uptake is receptormediated. It has been postulated that the site of insulin degradation following internalization is lysosomal. We have studied the uptake and cell processing of radiolabelled insulin using a combination of ex vivo liver perfusion and subcellular fractionation on sucrose density gradients. It appears that the processing of insulin is non-lysosomal. Various aspects of the processing pathway can be differentiated using weak bases: e.g. methylamine inhibits both uptake and processing whereas chloroquine affects only processing.
KeywordsSucrose HPLC Depression Fractionation NADH
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