Hepatic Processing of Insulin: Characterization and Modulation

  • G. D. Smith
  • J. R. Christensen
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 17)


The liver is a major target for insulin, up to 50% of the circulating insulin being taken up after a single pass. Uptake is receptormediated. It has been postulated that the site of insulin degradation following internalization is lysosomal. We have studied the uptake and cell processing of radiolabelled insulin using a combination of ex vivo liver perfusion and subcellular fractionation on sucrose density gradients. It appears that the processing of insulin is non-lysosomal. Various aspects of the processing pathway can be differentiated using weak bases: e.g. methylamine inhibits both uptake and processing whereas chloroquine affects only processing.


Sucrose Density Gradient Perfusion Medium Endocytic Vesicle Insulin Clearance Insulin Degradation 
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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • G. D. Smith
    • 1
  • J. R. Christensen
    • 1
  1. 1.Division of Clinical Cell BiologyMRC Clinical Research CentreHarrow, MiddlesexUK

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