Antibody Response in Swine to Individual Transmissible Gastroenteritis Virus (TGEV) Proteins
Transmissible gastroenteritis virus (TGEV) is a coronavirus that produces a life-threatening diarrhea in neonatal pigs. Whereas sows infected with virulent virus produce protective colostral antibody, sows vaccinated with attenuated virus are significantly less effective in passively protecting baby pigs. Therefore, an understanding of the antigenic and biochemical properties of pathogenic TGEV and the pigs’ immune response to these antigens is a critical area of TGEV research. We are in the process of characterizing the porcine immune response to major TGEV antigens in a protein A immunoprecipitation assay. Protein A is known to bind approximately 90% of the swine immunoglobulin isotypes, IgG1. and IgG2, but is less efficient in binding either swine IgM or IgA.1 This study has led to the serological identification of a new TGEV-specific low molecular weight polypeptide.
KeywordsSucrose Hepatitis EDTA Fluoride Electrophoresis
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