Advertisement

Use of Combined Monoclonal Antibodies for the Immunochemical Determination of Apolipoproteins A–I and B in Human Plasma

  • Santica Marcovina
  • Alberico L. Catapano
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 210)

Abstract

Plasma cholesterol level Is a risk factor for the development of coronary artery disease (1). Indeed, the cholesterol bound to low-density lipoprotein (LDL) appears to better correlate with the risk of coronary heart disease (CHD) as compared to plasma cholesterol levels while the cholesterol bound to high-density lipoprotein (HDL) is inversely related to the risk of developing CHD.

Keywords

Monoclonal Antibody Polyclonal Antiserum Familial Hypercholesterolemia Plasma Cholesterol Level Radial Immunodiffusion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1).
    Goldstein JL, Brown MS. Familial hypercholesterolemia. In Stanbury JB, Wyngaarden JB, Fredrickson DS, Goldstein JL, Brown MS, eds. The metabolic basis of inherited disease. New York, 672–712 (1983).Google Scholar
  2. 2).
    Avogaro P, Bittolo Bon G, Cazzolato G, Quinci GB, Belussi F. Plasma level of lipoprotein A-I and apolipoprotein B in human atherosclerosis. Artery 4, 385–394 (1978).Google Scholar
  3. 3).
    Riesen WF, Mordasini R, Salzmann C, Theler A., Gurtner HP. Apoproteins and lipids as discriminators of severity of coronary heart disease. Atherosclerosis 37, 157–162 (1980).PubMedCrossRefGoogle Scholar
  4. 4).
    De Backer G, Rosseneu M, Deslypere JP. Discriminative value of lipids and apoproteins in coronary heart disease. Atherosclerosis 42, 197–203 (1982).PubMedCrossRefGoogle Scholar
  5. 5).
    Köhler G, Milstein C. Continuous cultures of fused cells secreting antibodies of predifined specificity. Nature 256, 495–497 (1975).PubMedCrossRefGoogle Scholar
  6. 6).
    Tsao BP, Curtiss LK, Edgington TS. Immunochemical heterogeneity of human plasma apolipoprotein B. II. Expression of apolipoprotein B epitopes on native lipoproteins. J Biol Chem 257, 15222–15228 (1982).PubMedGoogle Scholar
  7. 7).
    Marcovina S, France D, Phillips RA, Mao SJT. Monoclonal antibodies can precipitate low-density lipoprotein. I. Characterization and use in determining apolipoprotein B. Clin Chem 31, 1654–1658 (1985).PubMedGoogle Scholar
  8. 8).
    Curtiss LK, Edgington TS. Immunochemical heterogeneity of human plasma high density lipoproteins. Identification with apolipoprotein A-I and A-II specific monoclonal antibodies. J Biol Chem 260, 2982–2993 (1985).PubMedGoogle Scholar
  9. 9).
    Marcovina S, Kottke BA, Mao SJT. Monoclonal antibodies can precipitate low density lipoprotein. II. Radioimmunoassay with single and combined monoclonal antibodies for determining apolipoprotein B in serum of patients with coronary artery disease. Clin Chem 31, 1659–1663 (1985).PubMedGoogle Scholar
  10. 10).
    Marcovina S, Di Cola G, Rapetto C, et al. Development of a radial immunodiffusion technique employing monoclonal antibodies for apolipoprotein B determination in human plasma. Clin Chim Acta 147, 117–125 (1985).PubMedCrossRefGoogle Scholar
  11. 11).
    Marcovina S, Price PJ, McDougal JS, Cremonesi L. Monoclonal antibodies directed against human low density lipoprotein and their characterization by the immunodot technique. Protides Biological Fluids Proc Colloq 31, 985–989 (1984).Google Scholar
  12. 12).
    Scanu AM, Edelstein C. Solubility in aqueous solutions of ethanol of the small molecular weight peptides of the serum very low density and high density lipoproteins. Relevance to the recovery problem during delipidation of serum lipoproteins. Analyt Biochem 53, 576–588 (1973).Google Scholar
  13. 13).
    Egusa G, Brady DW, Grundy SM, Howard BV. Isopropanol precipitation method for the determination of apolipoprotein B specific activity and plasma concentration during metabolic studies of very low density lipoprotein apolipoprotein B. J Lip Res 24, 1261–1267 (1983).Google Scholar
  14. 14).
    Markwell MAK, Haas SM, Bieber LL, Toebert NE. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem 87, 206–210 (1978).PubMedCrossRefGoogle Scholar
  15. 15).
    Wayne TF, Alaupovic P, Curry MD, et al. Plasma apolipoprotein B and VLDL-, LDL-, and HDL-cholesterol as risk factors in the development of coronary artery disease in male patients examined by angiography. Atherosclerosis 39, 411–424 (1981).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Santica Marcovina
    • 1
  • Alberico L. Catapano
    • 1
    • 2
  1. 1.S. Raffaele Scientific InstituteMilanItaly
  2. 2.Institute of Pharmacologycal SciencesMilanItaly

Personalised recommendations