Use of Combined Monoclonal Antibodies for the Immunochemical Determination of Apolipoproteins A–I and B in Human Plasma
Plasma cholesterol level Is a risk factor for the development of coronary artery disease (1). Indeed, the cholesterol bound to low-density lipoprotein (LDL) appears to better correlate with the risk of coronary heart disease (CHD) as compared to plasma cholesterol levels while the cholesterol bound to high-density lipoprotein (HDL) is inversely related to the risk of developing CHD.
KeywordsCholesterol EDTA Agarose Isopropanol Triglyceride
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- 1).Goldstein JL, Brown MS. Familial hypercholesterolemia. In Stanbury JB, Wyngaarden JB, Fredrickson DS, Goldstein JL, Brown MS, eds. The metabolic basis of inherited disease. New York, 672–712 (1983).Google Scholar
- 2).Avogaro P, Bittolo Bon G, Cazzolato G, Quinci GB, Belussi F. Plasma level of lipoprotein A-I and apolipoprotein B in human atherosclerosis. Artery 4, 385–394 (1978).Google Scholar
- 11).Marcovina S, Price PJ, McDougal JS, Cremonesi L. Monoclonal antibodies directed against human low density lipoprotein and their characterization by the immunodot technique. Protides Biological Fluids Proc Colloq 31, 985–989 (1984).Google Scholar
- 12).Scanu AM, Edelstein C. Solubility in aqueous solutions of ethanol of the small molecular weight peptides of the serum very low density and high density lipoproteins. Relevance to the recovery problem during delipidation of serum lipoproteins. Analyt Biochem 53, 576–588 (1973).Google Scholar
- 13).Egusa G, Brady DW, Grundy SM, Howard BV. Isopropanol precipitation method for the determination of apolipoprotein B specific activity and plasma concentration during metabolic studies of very low density lipoprotein apolipoprotein B. J Lip Res 24, 1261–1267 (1983).Google Scholar