Purine De Novo Synthesis and Salvage During Testicular Development in the Rat

  • Jennifer Allsop
  • Richard W. E. Watts
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 131)


We have encountered several patients with the Lesch-Nyhan syndrome in whom, although the testes were clinically normal in early childhood, they failed to develop at puberty and in one they could not be found at autopsy when he was 18 years old. This patient showed no evidence of pre-pubertal development although the plasma testosterone and gonadotrophin levels were consistent with approaching puberty and with his bone age of 13.2 years1. The testes of another clinically prepubertal Lesch-Nyhan syndrome patient who died at age 14 years showed tubules lined by simple germinal epithelium with no evidence of spermatogenesis. The interstitial tissue contained: numerous fibroblastic cells but no interstitial cells (Leydig cells) were seen.


Leydig Cell Plasma Testosterone Synthesis Activity Gonadotrophin Level Testicular Development 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    R.W.E. Watts, E. Spellacy, D.A. Gibbs, J. Allsop, R.O. McKeran and G.E. Slavin, Clinical, post-mortem, biochemical and therapeutic observations on the Lesch-Nyhan syndrome with particular reference to the neurological manifestations, Quart. Med., N.S., 51–43 (1982).Google Scholar
  2. 2.
    J. Allsop and R.W.E. Watts, Activities of amidophosphoribosyltrans-ferase and purine phosphoribosyltransferases in developing rat brain, Adv. exp. Biol. Med., 122A: 361 (1980).Google Scholar
  3. 3.
    J. Allsop and R.W.E. Watts, Purine synthesis and salvage in brain and liver, Adv. exp. Biol. Med., 165B: 21 (1984).Google Scholar
  4. 4.
    J. Allsop and R.W.E. Watts, Purine de novo synthesis in liver and developing rat brain, and the effect of some inhibitors of purine nucleotide interconversion, Enzyme, 30: 172 (1983)PubMedGoogle Scholar
  5. 5.
    J. Allsop and R.W.E. Watts, Activities of amidophosphoribosyltrans-ferase (EC and the purine phosphoribosyltransferases (EC and and the phosphoribosylpyrophosphate content of rat central nervous system at different stages of development, J. Neurol. Sci., 46: 221 (1980).PubMedCrossRefGoogle Scholar
  6. 6.
    O.H. Lowry, N.J. Rosebrough, A.L. Farr and R.J. Randall, Protein measurement with the Folin phenol reagent, J. biol. Chem., 193: 265 (1951).PubMedGoogle Scholar
  7. 7.
    R.W.E. Watts, Advances in Enzyme Regulation, Ed. G. Weber, 23 (1984) pp. 25–58.Google Scholar
  8. 8.
    W.N. Kelley, M.L. Greene, F.M. Rosenbloom, J.F. Henderson and J.E. Seegmiller, Hypoxanthine-guanine phosphoribosyltransferase deficiency in gout, Ann. Int. Med., 70: 155 (1969).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Jennifer Allsop
    • 1
  • Richard W. E. Watts
    • 1
  1. 1.Division of Inherited Metabolic DiseasesMRC Clinical Research CentreHarrowUK

Personalised recommendations