5-AZA-2′-Deoxycytidine Synergistic action with Thymidine on Leukemic Cells and Interaction of 5-AZA-dCMP with dCMP Deaminase
5-Aza-2′-deoxycytidine (5-AZA-CdR) is a potent antileukemic agent whose inhibitory effects are blocked by deoxycytidine (1-3). In order to be active, 5-AZA-CdR must first be phosphorylated by the enzyme deoxycytidine kinase (4). Leukemic cells deficient in the enzyme are resistant to 5-AZA-CdR (5). The lethal action of 5-AZA-CdR is related to its incorporation into DNA (6,7). The incorporation of 5-azacytosine analogs into DNA has been shown to induce the expression of differentiated phenotypes (8). This induction of differentiation by 5-AZA-CdR appears to be related to the inhibition of DNA methylation produced by these analogs (8, 9).
KeywordsHPLC Leukemia Pyrimidine Thymidine Dine
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