Alteration of Ribonucleotide and Deoxyribonucleotide Metabolism by Interferon in Human B- Lymphoblastoid Cells

  • Jerzy Barankiewicz
  • Chaim Kaplinsky
  • Amos Cohen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 131)


Inhibition of cell growth and proliferation by interferon has been studied intensively1-3. Although it was believed that the suppression of cell proliferation by interferon is associated with inhibition of thymidine kinase activity, recent studies using thymidine kinase deficient cells showed that activity of this enzyme is not crucial for interferon action4. Therefore, it was of great interest to study effects of interferon on other reactions of both ribonucleotide and deoxyribonucleotide metabolism, which possibly could explain inhibitory action of interferon on nucleic acid synthesis.


Thymidine Kinase Radioactive Substrate Orotic Acid Pyrimidine Metabolism Thymidine Kinase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    E. Knight Jr., Antiviral and cell growth inhibitory activities reside in the same glycoprotein of human fibroblast interferon, Nature (London) 262: 302–303 (1976).CrossRefGoogle Scholar
  2. 2.
    F. Balkwill, Cancer Immunol. Immunother. 7: 7–14, (1977).Google Scholar
  3. 3.
    I. Gresser, and M.G. Tovey, Biochem. Biophys. Acta 516: 231–247 (1978).PubMedGoogle Scholar
  4. 4.
    D.R. Gewert, A. Cohen, and B.R.G. Williams, The effect of interferon on cells deficient in nucleoside transport or lacking thymidine kinase activity, Biochem. Biophys. Res. Comm. 118: 124–130, (1984).PubMedCrossRefGoogle Scholar
  5. 5.
    J.F. Henderson, J.H. Fraser, and E.E. McCoy, Methods for the study of purine metabolism in human cells in vitro, Clin. Biochem. 7: 339–358 (1974).PubMedCrossRefGoogle Scholar
  6. 6.
    J.X. Khym, An analytical system for rapid separation of tissue nucleotides at low pressure on conventional anion exchange. Clin. Chem. 21: 1245–1251 (1975).PubMedGoogle Scholar
  7. 7.
    J. Barankiewicz and A. Cohen, Evidence for distinct catabolicpathways of adenine ribonucleotides and deoxyribonucleotides in human T lymphoblastoid cells. J. Biol. Chem. 259: 15178–15181 (1984).PubMedGoogle Scholar
  8. 8.
    A.S. Bagnara, L.W. Brox and J.F. Henderson, Kinetics of amidophosphoribosyltransferase in intact tumor cells. Biochem. Biophys. Acta 350: 171–182, 1974.PubMedCrossRefGoogle Scholar
  9. 9.
    D. Hunting, J. Hordern, and J.F. Henderson, Quantitative analysis of purine and pyrimidine metabolism in Chinese hamster ovary cells, Can. J. Biochem. 59: 838–847 (1981).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Jerzy Barankiewicz
    • 1
  • Chaim Kaplinsky
    • 1
  • Amos Cohen
    • 1
  1. 1.Division of Immunology Research InstituteThe Hospital for Sick ChildrenTorontoCanada

Personalised recommendations