Enzymatic Production of Secondary Metabolites
In a recent discussion of the microbiological production of Pharmaceuticals, it was pointed out that process improvement in the case of secondary metabolites can hardly be achieved by single gene manipulation, since between 10 and 30 structural genes contribute by the joint action of their products to the biosynthesis of one single complex compound.1 Reviewing the last 15 years progress on biosynthetic pathways with special reference to gene products or enzymes, we have found many instances of a new type of enzyme organization, i.e. one organized into multienzymes2 (table 1). A more detailed analysis shows that multienzymes may catalyze up to 8 consecutive reaction steps (table 2).
KeywordsBacillus Licheniformis Dissolve Oxygen Tension Dipeptide Cyclization Bacillus Polymyxa Continuous Procedure
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- 2.Kleinkauf, H., and Döhren, H. von (Eds.) (1982) Peptide Antibiotics-Biosynthesis and Functions. De Gruyter Berlin.Google Scholar
- 3.Kleinkauf, H., Döhren, H. von, Zocher, R., and Tesche, B. (1981).Google Scholar
- 5.Zharikova, G. G., Myaskovskaya, S. P., and Silaev, A. B. (1972) Vestn. Mosk. Univ. Biol. Puchvoved 27, 110Google Scholar
- 6.Kleinkauf, H., and Döhren, H. von (1983) in Biochemistry and Genetic Regulation of Commercially Important Antibiotics (L.C. Vining, ed.) pp. 95-145, Addison-Wesley, Reading.Google Scholar
- 8.Matteo, C. C., Cooney, C. L., and Demain, A.L. (1976) J. Gen Microbio1. 96, 415Google Scholar
- 9.Aust, H. J., and Döhren, H. von (1982) in Ref. 2. p. 137.Google Scholar
- 10.Chiu, C. W., Bernhard, T., and Dellweg, G. (1982) in Ref. 2, p. 149.Google Scholar