Enzymatic Production of Secondary Metabolites

  • H. Kleinkauf
  • H. V. Döhren
Part of the NATO ASI Series book series (NSSA, volume 87)

Abstract

In a recent discussion of the microbiological production of Pharmaceuticals, it was pointed out that process improvement in the case of secondary metabolites can hardly be achieved by single gene manipulation, since between 10 and 30 structural genes contribute by the joint action of their products to the biosynthesis of one single complex compound.1 Reviewing the last 15 years progress on biosynthetic pathways with special reference to gene products or enzymes, we have found many instances of a new type of enzyme organization, i.e. one organized into multienzymes2 (table 1). A more detailed analysis shows that multienzymes may catalyze up to 8 consecutive reaction steps (table 2).

Keywords

Glycerol Immobilization Proline Lysine Bacillus 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Hopwood, D. A., and Chater, K. T. (1980) Phil. Trans. Roy. Soc. London B 290, 313.PubMedCrossRefGoogle Scholar
  2. 2.
    Kleinkauf, H., and Döhren, H. von (Eds.) (1982) Peptide Antibiotics-Biosynthesis and Functions. De Gruyter Berlin.Google Scholar
  3. 3.
    Kleinkauf, H., Döhren, H. von, Zocher, R., and Tesche, B. (1981).Google Scholar
  4. 4.
    Hori, K., Kanda, M., Kurotsu, T., Miura, S., Yamada, Y., and Saito, Y. (1981) J. Biochem. 90, 439.PubMedGoogle Scholar
  5. 5.
    Zharikova, G. G., Myaskovskaya, S. P., and Silaev, A. B. (1972) Vestn. Mosk. Univ. Biol. Puchvoved 27, 110Google Scholar
  6. 6.
    Kleinkauf, H., and Döhren, H. von (1983) in Biochemistry and Genetic Regulation of Commercially Important Antibiotics (L.C. Vining, ed.) pp. 95-145, Addison-Wesley, Reading.Google Scholar
  7. 7.
    Tzeng, C. H., Thrasher, K. D., Montgomery, J. P., Hamilton, B.K. and Wang, D. I. C. (1975) Biotechnol. Bioeng. 17, 143CrossRefGoogle Scholar
  8. 8.
    Matteo, C. C., Cooney, C. L., and Demain, A.L. (1976) J. Gen Microbio1. 96, 415Google Scholar
  9. 9.
    Aust, H. J., and Döhren, H. von (1982) in Ref. 2. p. 137.Google Scholar
  10. 10.
    Chiu, C. W., Bernhard, T., and Dellweg, G. (1982) in Ref. 2, p. 149.Google Scholar
  11. 11.
    Tamaki, M., Takimoto, M., Sofuku, S., and Muramatsu, I. (1981) J. Antibiot. 34, 1227PubMedCrossRefGoogle Scholar
  12. 12.
    Tamaki, M., Takimoto, M., Sofuku, S., and Muramatsu, I. (1983) J. Antibiot. 36, 751PubMedCrossRefGoogle Scholar
  13. 13.
    Iwai, Y., and Omura, S. (1982) J. Antibiot. 35, 123PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • H. Kleinkauf
    • 1
  • H. V. Döhren
    • 1
  1. 1.Institut für Biochemie und Molekulare BiologieTechnischen Universität BerlinBerlin 10 (West)Germany

Personalised recommendations