Abstract
A sensitive technique (imprint electroimmunofixation, IEIF) for the electrophoretic characterization of virus-specific antibodies was developed: Serum and concentrated cerebrospinal fluid (CSF) are fractionated by agarose gel electrophoresis. Virus antigen-containing gel plates are then incubated in direct contact with the electrophoresis gel for 30 minutes. After separation of the gels the electrophoresis gel is fixed and stained with Coomassie Brilliant Blue. The antigen-containing gel is thoroughly washed in saline, incubated in a buffer containing I125-labelled rabbit anti-human Ig antibodies, washed again, dried and autoradiographed.
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© 1979 Plenum Press, New York
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Nordal, H.J., Vandvik, B., Norrby, E. (1979). Oligoclonal Virus Antibodies in Healthy Adults and Neurological Patients. In: Karcher, D., Lowenthal, A., Strosberg, A.D. (eds) Humoral Immunity in Neurological Diseases. NATO ASI Series, vol 24. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1003-7_39
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DOI: https://doi.org/10.1007/978-1-4684-1003-7_39
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-1005-1
Online ISBN: 978-1-4684-1003-7
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