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Use of Recombinant DNA Technology for the Production of Polypeptides

  • Chapter
Cell Substrates

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 118))

Abstract

There are many polypeptides of biologic and medical interest which have not been fully studied because they cannot be obtained in adequate quantity with sufficient purity and economy. Recent advances in recombinant DNA technology now give promise of in vivo synthesis in bacteria of a wide variety of polypeptide hormones, specific immunoglobins, enzymes and other proteins. This presentation will first review the procedures for constructing chimeric microorganisms containing the DNA coding for a eucaryotic protein, then discuss the problems and experience in obtaining such proteins from DNA cloned in procaryotic cells.

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Authors and Affiliations

  1. Endocrine Research Division and the Department of Pediatrics, University of California, San Francisco, USA

    Walter L. Miller M.D.

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  1. Walter L. Miller M.D.
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Editors and Affiliations

  1. Bureau of Biologics of the Food and Drug Administration, Bethesda, Maryland, USA

    John C. Petricciani  & Hope E. Hopps  & 

  2. W. Alton Jones Cell Science Center, Lake Placid, New York, USA

    Paul J. Chapple

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© 1979 Plenum Press, New York

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Miller, W.L. (1979). Use of Recombinant DNA Technology for the Production of Polypeptides. In: Petricciani, J.C., Hopps, H.E., Chapple, P.J. (eds) Cell Substrates. Advances in Experimental Medicine and Biology, vol 118. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-0997-0_16

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  • DOI: https://doi.org/10.1007/978-1-4684-0997-0_16

  • Publisher Name: Springer, Boston, MA

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  • Online ISBN: 978-1-4684-0997-0

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