Abstract
This report deals with the prospects for 13C NMR in defining the interactions of CO2 and its hydrated derivatives with small molecules and various proteins. The importance of bicarbonate ion as a buffer component is well recognized. The insolubility of many bicarbonate and carbonate salts is a central fact around which most people interested in metal-protein interactions try to work. The most common strategy is to remove or at least to avoid adding bicarbonate while setting up a metal-peptide or metal-protein study. The availability of 13C-enriched CO2 and bicarbonate and of NMR spectrometers capable of studying the 13C signals makes it convenient to reexamine the roles of these important interacting species in certain metal-protein systems of unquestionable importance.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bovey, F. A. (1969) Nuclear Magnetic Resonance Spectroscopy (Academic Press, New York), pp. 187–188.
Caplow, M. (1969) in Carbon Dioxide: Chemical, Biochemical and Physiological Aspects, eds. Forster, R. E., Edsall, J. T., Otis, A. G., and Roughton, F. J. W. (NASA SP-188, Washington, D. C.), pp. 47–52.
Dozy, A. M., Kleihaver, E. F., and Huisman, T. H. J. (1968) J. Chromatography 32, 723–727.
Garner, M. H., Garner, W. H., and Gurd, F. R. N. (1973) J. Biol. Chem. 248, 5451–545.
Gurd, F. R. N., Lawson, P. J., Cochran, D. W., and Wenkert, E. (1971) J. Biol. Chem. 246, 3725–3730.
Henriques, O. M. (1928) Biochem. Z. 200, 1–24.
Johncock, P., Kohnstam, G., and Speight, D. (1958) J. Chem. Soc. (London) 2544-2551.
Kilmartin, J. V., and Rossi-Bernardi, L. (1971) Biochem. J. 124, 31–45
Koenig, S. H., Brown, R. D., Needham, T. E., and Matwiyoff, N. A. (1973) Biochem. Biophys. Res. Comm. 53, 624–630.
Kroschwitz, J. I., Winokur, M., Reich, H. J., and Roberts, J. D. (1969) J. Am. Chem. Soc. 91, 5927–5928.
Lindskog, S., and Coleman, J. E. (1973) Proc. Nat. Acad. Sci. USA 70, 2505–2508.
Matsuda, G., Maita, T., and Nakagina, M. (1964) J. Biochem. (Tokyo) 56, 490–491.
Matwiyoff, N. A. and Needham, T. E. (1972) Biochem. Biophys. Res. Commun. 49, 1158–1164.
Morrow, J. S., Keim, P., and Gurd, F. R. N. (1974) in preparation.
Morrow, J. S., Keim, P., Visscher, R. B., Marshall, R. C., and Gurd, F. R. N. (1973) Proc. Nat. Acad. Sci. USA 70, 1414–1418.
Needleman, S. B., ed. (1970) Protein Sequence Determination, Springer-Verlag, New York, pp. 82–83.
Patterson, A., Jr., and Ettinger, R. (1960) Z. Elektrochem. 64, 98–110.
Rossi-Bernardi, L., Pace, M., Roughton, F. J. W., and Van Kempen, L. (1969) in Carbon Dioxide: Chemical, Biochemical and Physiological Aspects, eds. Forster, R. E., Edsall, J. T. Otis, A.G., and Roughton, F. J. W. (NASA SP-188, Washington, D. C.), PP. 65–71.
Roughton, F. J. W. (1964 in Handbook of Physiology, Section 3:Respiration, eds. Fenn, W. O. and Rahn, N. (American Physiological Society, Washington, D. C.), Vol. 1, p. 767.
Roughton, F. J. W., and Rossi-Bernardi, L. (1966) Proc. Roy. Soc. (London) B164, 381–400.
Shearer, W. T., Bradshaw, R. A., Gurd, F. R. N., and Peters, T. (1967) J. Biol. Chem. 242, 5451–5459.
Stadie, W. C., and O’Brien, H. (1936) J. Biol. Chem. 112, 723–758.
Stadie, W. C. and O’Brien, H. (1937) J. Biol. Chem. 117, 439–470.
Warner, R. C., and Weber, I. (1953) J. Amer. Chem. Soc. 75, 5094–5101.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1974 Plenum Press, New York
About this chapter
Cite this chapter
Gurd, F.R.N., Morrow, J.S., Keim, P., Visscher, R.B., Marshall, R. (1974). 13C NMR Studies of the Interaction of Hb and Carbonic Anhydrase with 13CO2 . In: Friedman, M. (eds) Protein-Metal Interactions. Advances in Experimental Medicine and Biology, vol 48. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-0943-7_6
Download citation
DOI: https://doi.org/10.1007/978-1-4684-0943-7_6
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-0945-1
Online ISBN: 978-1-4684-0943-7
eBook Packages: Springer Book Archive