Abstract
In our rheumatism research unit we are interested in the excretion of unconjugated corticosteroid metabolites because they may provide some evidence of the extra-hepatic metabolism of corticosteroids. A method was developed for estimating Cortisol metabolites in urine (1) which involved a preliminary separation of the extract by paper chromatography (Bush B5 system) and, after bismuthate oxidation, determination by GLC on a neopentyl glycol adipate column. This communication describes an improvement on the method and involves the following procedure.
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References
Bailey, E., J Endocr 28: 131, 1964
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© 1965 Plenum Press
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Bailey, E. (1965). The Estimation of Unconjugated Cortisol Metabolites in Urine by Gas Chromatography. In: Lipsett, M.B. (eds) Gas Chromatography of Steroids in Biological Fluids. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-0691-7_7
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DOI: https://doi.org/10.1007/978-1-4684-0691-7_7
Publisher Name: Springer, Boston, MA
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