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Steroid Hormone Receptors: Basic and Clinical Aspects pp 75–105Cite as

Estrogen Receptor Induced DNA Bending

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Part of the book series: Hormones in Health and Disease ((HHD))

Abstract

We have examined the ability of the full-length estrogen receptor (ER) and the purified estrogen receptor DNA binding domain to bend DNA on binding to estrogen response element (ERE). Purified ER DNA binding domain (DBD) expressed in E. coli, crude human ER expressed in MCF-7 cells, and partially purified human ER expressed in yeast, bound specifically to a series of circularly permuted ERE-containing DNA fragments. Binding of ER to a single ERE induced a reproducible DNA bend of 56°. This was 1.65 fold greater than the 34° bending angle induced by binding of the ER DNA binding domain. The DNA bending angle induced was the same whether the salt-extracted receptor was unoccupied, occupied by 17β-estradiol, or occupied by trans-hydroxytamoxifen. To determine if proteins associated with ER in MCF-7 cells affect the degree of bending, we examined the ability of partially purified human ER, expressed in yeast, to bend DNA. The degree of bending induced by the partially purified yeast ER, and by the crude MCF-7 cell ER, was the same. When two EREs were present in the DNA fragment, 1.2 and 1.55 fold increases in DNA bending were observed for the full-length ER, and for the ER DNA binding domain, respectively. Our demonstration that binding of human estrogen receptor to the ERE induces DNA bending suggests a role for DNA bending in ER-induced transcription activation.

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Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Illinois, USA

    David J. Shapiro

  2. The Ben May Institute, The University of Chicago, USA

    Geoffrey L. Greene

  3. Department of Physiology, University of Illinois, USA

    Ann M. Nardulli

Authors
  1. David J. Shapiro
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  2. Geoffrey L. Greene
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  3. Ann M. Nardulli
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Editor information

Editors and Affiliations

  1. Department of Biological Sciences, Oakland University, Rochester, Michigan, 48309-4401, USA

    V. K. Moudgil

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© 1994 Birkhäuser Boston

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Shapiro, D.J., Greene, G.L., Nardulli, A.M. (1994). Estrogen Receptor Induced DNA Bending. In: Moudgil, V.K. (eds) Steroid Hormone Receptors: Basic and Clinical Aspects. Hormones in Health and Disease. Birkhäuser Boston. https://doi.org/10.1007/978-1-4615-9849-7_3

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  • DOI: https://doi.org/10.1007/978-1-4615-9849-7_3

  • Publisher Name: Birkhäuser Boston

  • Print ISBN: 978-1-4615-9851-0

  • Online ISBN: 978-1-4615-9849-7

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