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Chemical Synthesis of Oligodeoxyribonucleotides

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Techniques in Molecular Biology

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Abstract

Oligodeoxyribonucleotides of defined sequence may now be synthesised as a matter of routine at a reasonable cost in both time and materials. As both applications of and synthetic methods for oligodeoxyribonucleotides are much more highly developed than those for oligoribonucleotides, the term ‘oligonucleotide’ has come to be synonymous with oligodeoxyribonucleotide and will be used in this way in this chapter. An oligonucleotide of 15–35 nucleotides may be synthesised and purified in 3–5 days using simple apparatus and well-established methodologies. Slightly more sophisticated approaches allow the ‘simultaneous’ synthesis of 2 to about 125 oligonucleotides in a similar time.

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Further Reading

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Authors
  1. H. A. White
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Editors and Affiliations

  1. Division of Biological and Environmental Sciences, The Hatfield Polytechnic, PO Box 109, College Lane, Hatfield, Hertfordshire, AL10 9AB, England

    John M. Walker (Principal Lecturer in Biochemistry) (Principal Lecturer in Biochemistry)

  2. Department of Infectious Diseases, Section of Bacteriology, University of Utrecht, PO Box 80.171, 3508 TD, Utrecht, The Netherlands

    Wim Gaastra (Associate Professor of Microbiology) (Associate Professor of Microbiology)

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© 1987 John M. Walker and Wim Gaastra

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White, H.A. (1987). Chemical Synthesis of Oligodeoxyribonucleotides. In: Walker, J.M., Gaastra, W. (eds) Techniques in Molecular Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9799-5_13

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  • DOI: https://doi.org/10.1007/978-1-4615-9799-5_13

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-7099-3673-2

  • Online ISBN: 978-1-4615-9799-5

  • eBook Packages: Springer Book Archive

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