Abstract
In many situations it is very convenient to express a gene in a variety of different cell types, other than those from which it was derived. This could be useful in a number of areas such as the study of the regulation of gene expression and the biological function of gene products. There are a number of methods available (outlined below) to transfer both cloned and uncloned genes. Unfortunately, there is no method suitable for all situations and one must choose a particular method to suit the genetic material being used, the type of study to be undertaken and, most importantly, the cell type under study. Suggestions for suitable usage are made in each section. The calcium phosphate precipitation method1 is the one most widely used because it is suitable for most forms of DNA and is technically very simple to perform. Consequently, this method is described in most detail. Other methods which require sophisticated apparatus (such as microinjection) or great expertise (such as viral transformation) are described in less detail, but give essential references for those whose needs are suited to these methods.
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Further Reading
Hogan, B.L.M., Costantini, F. and Lacy, E. (eds) (1986) Manipulation of the Mouse Embryo: A Laboratory Manual (Cold Spring Harbor Laboratory)
Maniatis, T., Fritsch, E.F. and Sambrook, J. (eds) (1982) Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory)
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© 1987 John M. Walker and Wim Gaastra
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James, R.F.L., Grosveld, F.G. (1987). DNA-Mediated Gene Transfer into Mammalian Cells. In: Walker, J.M., Gaastra, W. (eds) Techniques in Molecular Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9799-5_11
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DOI: https://doi.org/10.1007/978-1-4615-9799-5_11
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