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Techniques in Molecular Biology pp 187–202Cite as

DNA-Mediated Gene Transfer into Mammalian Cells

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Abstract

In many situations it is very convenient to express a gene in a variety of different cell types, other than those from which it was derived. This could be useful in a number of areas such as the study of the regulation of gene expression and the biological function of gene products. There are a number of methods available (outlined below) to transfer both cloned and uncloned genes. Unfortunately, there is no method suitable for all situations and one must choose a particular method to suit the genetic material being used, the type of study to be undertaken and, most importantly, the cell type under study. Suggestions for suitable usage are made in each section. The calcium phosphate precipitation method1 is the one most widely used because it is suitable for most forms of DNA and is technically very simple to perform. Consequently, this method is described in most detail. Other methods which require sophisticated apparatus (such as microinjection) or great expertise (such as viral transformation) are described in less detail, but give essential references for those whose needs are suited to these methods.

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Further Reading

  • Hogan, B.L.M., Costantini, F. and Lacy, E. (eds) (1986) Manipulation of the Mouse Embryo: A Laboratory Manual (Cold Spring Harbor Laboratory)

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  • Maniatis, T., Fritsch, E.F. and Sambrook, J. (eds) (1982) Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory)

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Authors
  1. Roger F. L. James
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  2. Frank G. Grosveld
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Editor information

Editors and Affiliations

  1. Division of Biological and Environmental Sciences, The Hatfield Polytechnic, PO Box 109, College Lane, Hatfield, Hertfordshire, AL10 9AB, England

    John M. Walker (Principal Lecturer in Biochemistry) (Principal Lecturer in Biochemistry)

  2. Department of Infectious Diseases, Section of Bacteriology, University of Utrecht, PO Box 80.171, 3508 TD, Utrecht, The Netherlands

    Wim Gaastra (Associate Professor of Microbiology) (Associate Professor of Microbiology)

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© 1987 John M. Walker and Wim Gaastra

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James, R.F.L., Grosveld, F.G. (1987). DNA-Mediated Gene Transfer into Mammalian Cells. In: Walker, J.M., Gaastra, W. (eds) Techniques in Molecular Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9799-5_11

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  • DOI: https://doi.org/10.1007/978-1-4615-9799-5_11

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-7099-3673-2

  • Online ISBN: 978-1-4615-9799-5

  • eBook Packages: Springer Book Archive

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