Abstract
The great complexity of the mammalian central nervous system (CNS) has made it difficult to elucidate the cellular mechanisms involved in its development and function. Primary culture of the various cells, which are derived from tissue taken directly from the organism, therefore presents an attractive approach for investigating the biochemical, morphological and functional aspects of these cells during differentiation (1). In addition, the direct effect of toxic agents can be evaluated, including the mechanism of action on specific cell types.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
E. Hansson, Primary astroglial cultures. A biochemical and functional evaluation, Neurochem.Res. 11: 759 (1986).
A.P. Streissguth, S. Landesman-Dwyer, J.C. Martin and D.W. Smith, Teratogenic effects of alcohol in humans and laboratory animals, Science, 209: 353 (1980).
C. Guerri, A. Esquifino, R. Sanchis and S. Grisolla, Growth, enzymes and hormonal changes in offspring of alcohol-fed rats, in: “The Mechanisms of Alcohol Damage in Utero”, (Ciba Foundation), pp. 81, London. (1985).
M.W. Miller, Effects of alcohol on the generation and migration of cerebral cortical neurons, Science. 233: 1308 (1986).
J. Renau-Piqueras, R. Zaragoza, P.De Paz, R. Baguena, L. Megias and C. Guerri, Effects of prolonged ethanol exposure on the glial fibrillary acidic protein-containing intermediate filaments of astrocytes in primary culture: A quantitative immunofluorescence and immunogold electron microscopic study, J.Histochem.Cytochem. 37: 229 (1989)
J. Renau-Piqueras, M. Sancho-Tello, R. Zaragoza and C. Guerri, Ethanol on the development of astrocytes in primary culture, in: “Alcohol Toxicity and Free Radicals”, R. Nordmann, C. Ribiere and H. Rouach, eds., pp. 269, Pergamon, Oxford. (1988).
L. Hertz, Astrocytes, in: “Handbook of Neurochemistry”, A. Lajtha, ed., pp. 319, Plenum Press,New York. (1982).
L. Lazarides, Intermediate filaments: a chemically heterogeneous developmental1y regulated class of proteins, Ann.Rev.Biochem. 51: 219 (1982).
K. Hallermayer, C. Harmening and B. Hamprecht, Cellular localization and regulation of glutamine synthetase in primary cultures of brain cells from newborn mice, J.Neurochem. 37: 43 (1981).
M. Sensenbrenner, G.Devi Hers, E. Bock and A. Porte, Biochemical and ultrastructural studies of cultures rat astrogial cells. Effect of brain extract and dibutyril cyclic AMP on glial fibrillary acidic protein and glial filaments, Differentiation, 17: 51 (1980).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Plenum Press, New York
About this chapter
Cite this chapter
Guerri, C., Sancho-Tello, M., Zaragoza, R., Renau-Piqueras, J. (1990). The Use of Primary Culture of Astrocytes to Study Glial Development. Effect of Ethanol. In: Cuezva, J.M., Pascual-Leone, A.M., Patel, M.S. (eds) Endocrine and Biochemical Development of the Fetus and Neonate. Reproductive Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9567-0_21
Download citation
DOI: https://doi.org/10.1007/978-1-4615-9567-0_21
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4615-9569-4
Online ISBN: 978-1-4615-9567-0
eBook Packages: Springer Book Archive