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Color Analysis Method for Studying Oxygen Transport in Hemoglobin Solutions Using an Image-Input and -Processing System

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Oxygen Transport to Tissue X

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 222))

Abstract

Since Scholander (1960) and Wittenberg (1959) found that the diffusion of oxygen in aqueous solution is facilitated in the presence of hemoglobin, its dynamic behavior has been investigated extensively by many researchers (Hemmingsen and Scholander, 1960; Hemmingsen, 1962; Wittenberg, 1966; Kutchai and Staub, 1969; Spaan, Kreuzer, and Wely, 1980). The phenomena were observed not only in the steady state of oxygen transport but also in the transient state to equilibrium. Scholander and Wittenberg wetted a Millipore membrane with hemoglobin solution and estimated the membrane permeability to oxygen using a gas analysis technique. On the other hand, Spaan et al. measured the oxygenation rate of hemoglobin by spectrophotometry when oxygen was flushed onto the surface of the thin-layered hemoglobin solution. In both cases, the enhanced transport of oxygen by hemoglobin was observed.

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Author information

Authors and Affiliations

  1. Department of Physico-chemical Physiology, Osaka University Medical School, Osaka 530, Japan

    Masafumi Hashimoto, Ryuji Hata & Itiro Tyuma

  2. Department of General Education Kinki University, Higashiosaka 577, Japan

    Akio Isomoto

  3. Osaka Prefectural Institute of Public Health, Osaka 537, Japan

    Mitsuro Uozumi

Authors
  1. Masafumi Hashimoto
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  2. Ryuji Hata
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  3. Itiro Tyuma
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  4. Akio Isomoto
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  5. Mitsuro Uozumi
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Editor information

Editors and Affiliations

  1. Nishimaruyama Hospital, Sapporo, Japan

    Masaji Mochizuki

  2. The University of Rochester, Rochester, New York, USA

    Carl R. Honig

  3. Hokkaido University, Sapporo, Japan

    Tomiyasu Koyama

  4. Northwestern University, Evanston, Illinois, USA

    Thomas K. Goldstick

  5. California Polytechnic State University, San Luis Obispo, California, USA

    Duane F. Bruley

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© 1988 Plenum Press, New York

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Hashimoto, M., Hata, R., Tyuma, I., Isomoto, A., Uozumi, M. (1988). Color Analysis Method for Studying Oxygen Transport in Hemoglobin Solutions Using an Image-Input and -Processing System. In: Mochizuki, M., Honig, C.R., Koyama, T., Goldstick, T.K., Bruley, D.F. (eds) Oxygen Transport to Tissue X. Advances in Experimental Medicine and Biology, vol 222. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-9510-6_27

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  • DOI: https://doi.org/10.1007/978-1-4615-9510-6_27

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4615-9512-0

  • Online ISBN: 978-1-4615-9510-6

  • eBook Packages: Springer Book Archive

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